皂角米多糖的提取、改性及其寡糖的抗氧化活性分析
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(1.河北农业大学食品科技学院 河北保定 071000;2.河北农业大学林学院 河北保定 071000)

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国家自然科学基金项目(31901631);河北省重点研发计划项目(19226808D)


Extraction and Modification of Gleditsia sinensis Lam. Seed Polysaccharides and the Antioxidant Activity of Oligosaccharides
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(1.College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, Hebei;2.College of Forestry, Hebei Agricultural University, Baoding 071000, Hebei)

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    摘要:

    皂角米多糖是一种新型功能多糖,在食品工业中应用潜力巨大。采用水提醇沉法提取皂角米多糖,探讨非均相湿热改性技术对皂角米多糖的理化性质、寡糖制备效率和抗氧化活性的影响。结果表明:从皂角米中获得多糖,提取率为17.4%。经非均相湿热技术改性后,皂角米多糖溶液(10 g/L)表观黏度显著降低,由834 mPa·s降至97 mPa·s;热稳定性显著提高,且多糖微观形貌变得更光滑、平整,粒径显著变小,比表面积增大为0.245 m2/g。改性后多糖的水解率(40 g/L)提高了17.4%,且低分子质量(<5 ku)的寡糖产物含量显著增加,总抗氧化活性明显增强。结论:非均相湿热技术可用于皂角米多糖的寡糖的制备,研究结果可为皂角米的开发应用提供一定的理论参考。

    Abstract:

    The Gleditsia sinensis Lam. seed polysaccharides is a new type of functional polysaccharide with great potential for application in the food industry. In this study, the water extraction and alcohol precipitation method was used to extract the Gleditsia sinensis Lam. seed polysaccharide, and the influence of heterogeneous hygrothermal modification technology on its properties, oligosaccharide preparation efficiency and antioxidant activity was discussed. In this work, polysaccharides was obtained from the seed of Gleditsia Sinensis Lam. with an extraction rate of 17.4%. It was found that the apparent viscosity of the polysaccharide solution (10 g/L) decreased significantly after being modified, from 834 mPa·s to 97 mPa·s; the thermal stability was significantly improved, and the microscopic morphology became smoother and flatter, the particle size was significantly smaller, and the specific surface area increased to 0.245 m2/g. Moreover, the hydrolysis rate of modified polysaccharides (40 g/L) increased by 17.4%, and the content of low molecular weight (<5 ku) oligosaccharides was significantly increased, and the total antioxidant activity was significantly enhanced. These results indicated that the heterogeneous hygrothermal modification technology was beneficial to the preparation of the Gleditsia sinensis Lam. poligosaccharides, and could provide a certain theoretical support for the development and application of the Gleditsia Sinensis Lam.

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刘学,卢海强,李保会,王玉印,田洪涛,谷新晰.皂角米多糖的提取、改性及其寡糖的抗氧化活性分析[J].中国食品学报,2023,23(5):69-77

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  • 收稿日期:2022-05-19
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  • 在线发布日期: 2023-06-25
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