Patulin (4-hydroxy-4H-fruo[3，2-c]pran-2[6H]-one) is a naturally occurring mycotoxin produced by a number of fungal including Aspergillus， Byssochlamys， Penicillium， Mucor， and Rhizopus， and contaminate a wide variety of food products such fruit products， vegetables， grains and cheeses. The conventional method for patulin detection required separation and concentration process with organic solvent which is environmentally harmful. Considering the limitation of the traditional methods， this study developed a rapid， sensitive and environmentally friendly method for detection of patulin by using of a rabbit antibody against patulin and antibody conjugated immunolipsome. The detection limit of developed assay had detection limit of 8 μg/L without the need of organic solvent for extraction， separation， and purification. Overall， the detection limit meet well of European regulatory limit for infants and young children products (10 μg/L). The developed method was rapid， sensitive， environment-friendly， and reliable for the detection of patulin in apple juice.