With the widespread recognition of the health benefits of probiotic lactic acid bacteria， the probiotic industry has ushered in a golden period of prosperity. Nevertheless， the development of China's probiotics industry is lagged by lack of independent intellectual property rights for probiotic strains and outdated screening and industrialization technologies. At present， China mainly relies on traditional pure culture techniques for lactic acid bacteria isolation， which is time-consuming， labor-intensive， and has limited accuracy. However， in recent years， the rise of high-throughput sequencing and artificial intelligence technology has opened a new chapter in the screening of probiotic lactic acid bacteria. Based on genome Big Data and artificial intelligence， researchers have developed precise isolation and identification technology for lactic acid bacteria， significantly improving the screening speed and accuracy， and also promoting the industrialization of probiotics. This article will comprehensively review the precise isolation and industrialization technology of probiotic lactic acid bacteria based on artificial intelligence， and further explore the development trend of China's probiotics industry.