发芽绿豆多酚提取物对Ⅱ型糖尿病小鼠糖脂代谢的改善作用
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(1.黑龙江省农业科学院食品加工研究所 黑龙江省食品加工重点实验室黑龙江省全谷物营养食品工程技术研究中心 哈尔滨 150086;2.黑龙江八一农垦大学 黑龙江大庆 163000)

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“十四五”国家重点研发计划项目(2021YFD2100902)


The Ameliorative Effect of Polyphenol Extract from Sprouting Mung Bean on Glycolipid Metabolism in Type 2 Diabetic Mice
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(1.Food Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Heilongjiang Province Key Laboratory of Food Processing, Heilongjiang Province Engineering Research Center of Whole Grain Nutritious Food,Harbin 150086;2.Heilongjiang Bayi Agricultural Reclamation University, Daqing 163000, Heilongjiang)

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    摘要:

    目的:探究发芽绿豆多酚提取物对Ⅱ型糖尿病(T2DM)模型小鼠糖脂代谢的改善作用。方法:根据C57BL/6小鼠体重随机分为正常对照组、模型组、发芽绿豆多酚提取物高、中、低剂量组、阳性对照组。通过高脂饮食联合腹腔注射链脲佐菌素(STZ)的方法建立2型糖尿病小鼠模型。连续灌胃5周后,测定小鼠空腹血糖值、葡萄糖耐量、脏器指数、血脂水平等指标,观察肝脏组织形态。结果:与模型组相比,发芽绿豆多酚提取物能降低小鼠空腹血糖,并显著提高其糖耐量水平(P<0.01);改善T2DM小鼠血清中血脂指标(TC、TG、LDL-C、HDL-C)的浓度(P<0.01);降低小鼠血清ALT、AST的活力,并对STZ诱导的糖尿病小鼠肝脏组织细胞形态有一定的修复作用。结论:一定剂量的发芽绿豆多酚提取液可以有效改善Ⅱ型糖尿病小鼠高血糖和血脂代谢紊乱的症状。

    Abstract:

    Objective: To investigate the improvement of glycolipid metabolism in mice with type 2 diabetes mellitus (T2DM) induced by polyphenol extract from mung bean sprout. Methods: C57BL/6 mice were randomly divided into normal control group, model group, high, medium and low dose groups of germinated mung bean polyphenol extract and positive control group. A mouse model of type 2 diabetes was established by high fat diet combined with intraperitoneal injection of Streptozotocin (STZ). After continuous gavage for 5 weeks, fasting blood glucose, glucose tolerance, organ index, blood lipid level and liver morphology were measured. Results: during the experiment, compared with the model group, the polyphenol extract of germinated mung bean could reduce the fasting blood glucose and significantly improve the glucose tolerance of mice (P<0.01); Improve the concentration of serum lipid indexes (TC, TG, LDL-C, HDL-C) in T2DM mice(P<0.01); The activity of ALT and AST in serum of mice was reduced, and the morphology of liver tissue in STZ induced diabetic mice was repaired. Conclusion: a certain dose of germinated mung bean polyphenols extract can effectively improve the symptoms of hyperglycemia and dyslipidemia in type 2 diabetic mice.

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沈鑫婷,姜秀杰,许庆鹏,张东杰.发芽绿豆多酚提取物对Ⅱ型糖尿病小鼠糖脂代谢的改善作用[J].中国食品学报,2023,23(6):132-139

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  • 收稿日期:2022-06-08
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  • 在线发布日期: 2023-08-17
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