基于Illumina高通量测序分析相温贮藏对黄花菜表面微生物的影响
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(1.天津市农业科学院农产品保鲜与加工技术研究所 天津 300384;2.国家农产品保鲜工程技术研究中心(天津) 农业农村部农产品贮藏保鲜重点实验室天津市农产品采后生理与贮藏保鲜重点实验室 天津 300384;3.辽宁大学轻型产业学院 沈阳 110036)

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甘肃省科技计划重大项目(21ZD4NA016);天津市科技计划项目(21ZYCGSN00300)


The Effect of Phase Temperature Storage on the Surface Microorganisms of Daylily Based on Illumina High-throughput Sequencing Technology
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(1.Institute of Agricultural Products Preservation and Processing Technology, Tianjin Academy of Agricultural Sciences, Tianjin 300384;2.Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, Key Laboratory of Storage of Agricultural Products, Ministry of Agriculture and Rural Affairs, National Engineering and Technology Research Center for Preservation of Agricultural Products (Tianjin), Tianjin 300384);3.College of Light Industry, Liaoning University, Shenyang 110036)

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    摘要:

    研究相温贮藏对黄花菜表面微生物种群结构的影响,为黄花菜保鲜提供参考。将采自甘肃庆阳的黄花菜分组包装后分别放入冰箱(4 ℃±1 ℃)、冷库(0 ℃±0.5 ℃)及相温环境(-0.5 ℃±0.1 ℃)中贮藏,7 d时测褐变率,并提取表面微生物样本,利用特异性的引物分别对细菌宏基因组16S rRNA的V3-V4区和真菌宏基因组ITS1-1F区进行PCR扩增,基于Illumina高通量测序技术分析黄花菜微生物相对丰度及多样性。在属水平上,座囊菌、小葡萄菌、银耳菌、散囊菌、子囊菌是黄花菜真菌群落的优势菌群,蓝细菌、γ-变形杆菌、α-变形杆菌、芽孢杆菌、梭菌是黄花菜细菌群落的优势菌群。从稀释曲线的OTU数量来看,相温贮藏黄花菜表面真菌及细菌多样性均为最低,表明相温贮藏可有效抑制表面微生物的多样性生长。

    Abstract:

    The research on the influence of phase temperature storage technology on the microbial population structure on the surface of daylily provides a reference for the preservation of daylily. The daylily collected from Qingyang, Gansu was packaged in groups and stored in the refrigerator (4 ℃±1 ℃), cold storage (0 ℃±0.5 ℃) and phase temperature environment (-0.5 ℃±0.1 ℃). The browning was measured at 7 days, and extracting surface microbial samples, using specific primers to perform PCR amplification on the V3-V4 region of the bacterial metagenomic 16S rRNA and the fungal metagenomic ITS1-1F region, analyzing the microbial relative abundance and diversity of daylily based on Illumina high-throughput sequencing technology. At the genus level, Dothideomycetes, Microbotryomycetes, Tremellomycetes, Eurotiomycetes, and Sordariomycetes are the dominant flora of daylily fungal communities, and Cyanobacteriia, Gammaproteobacteria, Alphaproteobacteria, Bacilli and Clostridia are the dominant flora of the daylily bacterial community. From the perspective of the number of OTUs in the dilution curve, the diversity of fungi and bacteria on the surface of daylily stored at phase temperature is the lowest, which proves that storage at phase temperature can effectively inhibit the diverse growth of surface microorganisms.

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张鹏,刘英杰,贾晓昱,薛友林,李江阔.基于Illumina高通量测序分析相温贮藏对黄花菜表面微生物的影响[J].中国食品学报,2023,23(6):285-293

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  • 收稿日期:2022-06-14
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  • 在线发布日期: 2023-08-17
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