AI-2 signal molecule could regulate the biofilm formation and probiotic property of lactic acid bacteria (LAB). In this study， the strain with high production AI-2 were screened from 16 strain lactic acid bacteria derived from traditional fermented food and carp intestines. The ability of biofilm formation， antioxidant and aroma-producing of high production AI-2 LAB were analyzed. The results showed that the relative fluorescence intensity of the high production AI-2 strain DBM2-4 was 9.69. The strain DBM2-4 was identified as Lactiplantibacillus plantarum by physiological and biochemical and 16S rRNA sequencing analysis. Compared with the low production AI-2 strain YF-7， strain DBM2-4 was cultured at 37 ℃ for 24， 48 h， and 72 h， the production of biofilm formation increased by 85.48%， 135.54%， 63.03%， respectively. Meanwhile， the free radical scavenging capacity of DPPH for complete cell and cell-free extracts of strain CY1-2 increased by 24.49% and 11.37%. The production of diacetyl and acetaldehyde increased by 3.42 mg/L and 5.86 mg/L， respectively. These results indicated that AI-2 signal molecule regulated the biofilm formation， antioxidant activity and aroma-producing activity of Lactiplantibacillus plantarum DBM2-4， The screening of LAB with high production of AI-2 would provide a theoretical basis for improving the probiotic， antioxidant and aroma-producing activity of LAB starters.