In this work， Litopenaeus vannamei was used as a raw material to study the changes of muscle protein quality characteristics at different storage times under partial freezing condition (-3 ℃)， and the radial basis function neural prediction model was established based on related protein indexes. The results showed that compared with fresh shrimp samples， after 30 d of partial freezing storage， the surface hydrophobicity， carbonyl content， TCA soluble peptide， myofibril fiber fragmentation index， hydrophobic interaction force， and disulfide bond content significantly increased to (68.12 ± 0.19) μg， (2.02 ± 0.08) nmol/mg pro， (6.29 ± 0.14) μmol tyrosine/g muscle， 55.11 ± 2.79， (7.82 ± 0.17) mg/mL， (5.77 ± 0.06) mg/mL， respectively. Ca2+-ATPase， total sulfhydryl content， ionic bonding， hydrogen bonding content， and endogenous fluorescence intensity significantly decreased to (0.13 ± 0.13) U/mg pro， (68.45 ± 0.47) μmol/g pro， (3.12 ± 0.48) mg/mL， (1.67 ± 0.14) mg/mL， 579.71 ± 20.67， respectively. Shrimp muscle proteins were denatured， oxidized and degraded in varying degrees， and the tertiary structure of proteins changed. The results of sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed that the storage time was prolonged， and the large molecular weight proteins were degraded. Meanwhile， electrophoresis results showed that the storage time was prolonged and the large molecular weight protein was degraded. The error between the predicted value of the radial basis function neural prediction model and the experimental value was within ±10%. The results showed that the model could predict the protein quality of L.vannamei during partial freezing storage.