酸菜发酵过程中细菌群落结构变化及驱动机制
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(太原师范学院生物系 山西晋中 030012)

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山西省科学技术厅自然基金面上项目(202303021211119)


The Changes and Driving Mechanism of Bacterial Community Structure during Paocai Fermentation
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(Department of Biology, Taiyuan Normal University, Jinzhong 030012, Shanxi)

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    摘要:

    酸菜质量的标准化依赖于其发酵过程中的微生物群落结构的动态演替。本研究利用高通量测序技术研究酸菜发酵过程细菌群落结构组成的动态变化,以及理化因子的动态变化规律,揭示发酵环境与微生物群落结构之间的相关性,并进行功能预测和代谢通路分析。结果表明:酸菜发酵初期、中期和末期三阶段中初期S1阶段的Simpson指数显著高于S2、S3阶段(P<0.05),Shannon指数显著低于S2、S3阶段(P<0.05),表明发酵初期细菌群落的多样性较低。从9个酸菜样品中检测到的细菌种类隶属于6门、9纲、29目、47科、59属、83种。酸菜发酵中主要的优势菌门是厚壁菌门和变形菌门,假单胞菌、乳杆菌、肠杆菌、乳球菌为优势属。细菌群落结构在不同阶段具有明显差异,在发酵初期、中期和末期,厚壁菌门的相对丰度分别是10.61%,64.83%,77.51%,而变形菌门由84.18%降至33.19%,最终降到17.91%。假单胞菌属相对丰度由发酵初期65.36%,发酵中期21.21%降到发酵后期的7.10%,乳杆菌属由相对丰度小于1%,到发酵中期迅速增至58.06%,再到发酵后期增至67.65%。曲度乳杆菌是发酵中期的主要乳酸菌,植物乳杆菌在发酵后期占据主要优势。通过相关性分析和冗余分析发现,可滴定酸和亚硝酸是引起细菌群落结构变化的主要驱动因子,对细菌群落组成影响最大。亚硝酸盐与发酵初期群落呈正相关关系,氨基酸态氨主要影响发酵中期的细菌群落,可溶性蛋白和可滴定酸对发酵后期的群落结构影响较大。可滴定酸与乳杆菌属呈显著正相关关系,与假单胞菌属呈显著负相关关系,乳酸菌可通过乳酸发酵创造的酸性环境显著抑制假单胞菌等有害微生物的生长。本研究阐明了自然发酵酸菜在不同阶段的群落组成及差异,并说明影响其组成的驱动因子,为酸菜的生产提供理论支持。

    Abstract:

    The standardization of paocai quality depends on the microbial community structure in the fermentation process. In this study, high-throughput sequencing technology was used to study the dynamic changes of bacterial community structure composition, detect the dynamic changes of physical and chemical factors, reveal the correlation between them, and carry out functional prediction and metabolic pathway analysis. The results showed that: Simpson index in S1 stage was significantly higher than that in S2 and S3 stage, while Shannon index was significantly lower than that in S2 and S3 stage (P<0.05), indicating that the diversity of bacterial community was low in the early fermentation stage. The bacterial species detected from 9 samples of paocai belong to 6 phylum, 9 classes, 29 orders, 47 families, 59 genera, 83 species. Firmicutes and Proteobacteria were the dominant phyla in paocai fermentation. The dominant genera were Pseudomonas, Lactobacillus, Enterobacteriaceae and Lactococcus. The relative abundance of Firmicutes was 10.61%, 64.83% and 77.51% at the beginning, middle and end of fermentation respectively, while Proteobacteria decreased from 84.18% to 17.91%, and Pseudomonas decreased from 33.19% at the beginning of fermentation. The relative abundance of Lactobacillus increased rapidly from less than 1% to 67.65% in the end fermentation period. L. curvatus was the main Lactobacillus in the middle fermentation period, L. plantarum was the main Lactobacillus in the late fermentation period. Correlation analysis and Redundancy analysis showed that titratable acid and nitrite were the main driving factors of bacterial community structure change, and had the greatest influence on bacterial community composition. Nitrite was positively correlated with the community at the early fermentation stage, amino acid ammonia mainly affected the community at the middle fermentation stage, soluble protein and TTA had a greater impact on the community structure at the late fermentation stage. Titratable acids were significantly positively correlated with Lactobacillus and negatively correlated with Pseudomonas. The acidic environment created by lactic acid bacteria through lactic acid fermentation could significantly inhibit the growth of Pseudomonas and other harmful microorganisms. In this study, the community composition and differences of naturally fermented paocai in different stages were elucidated, and the driving factors affecting its composition were explained, which provided help for the production of paocai.

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贾晶晶,赵虎威,燕平梅.酸菜发酵过程中细菌群落结构变化及驱动机制[J].中国食品学报,2023,23(8):354-368

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  • 收稿日期:2023-01-23
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  • 在线发布日期: 2023-09-01
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