Objective: The way of eating has an important influence on the intestinal immunity and inflammatory environment. This article examines the effects of high-sugar and high-fat diet (HSFD) on the intestinal barrier function and the level of inflammation. Methods: In the experiment， SPF grade C57BL/6J mice were selected for HSFD intervention for 4 weeks. At the same time， low-sugar and low-fat diet (LFD) was used as a control to determine blood lipid-related indicators， intestinal permeability， inflammation levels， and related tight junction protein expression changes. Results: Under HSFD conditions， the total cholesterol (TC)， low-density lipoprotein cholesterol (LDL-C)， triglyceride (TG) and other blood lipid indexes of mice increased significantly (P<0.05)， and high-density lipoprotein cholesterol (HDL-C) The content was significantly reduced(P<0.01)， and the pathological staining of the liver showed abnormal accumulation of adipocytes， indicating that the intervention of HSFD caused the disorder of lipid metabolism in the liver of mice. Further analysis of the symptoms of colon inflammation in mice showed that HSFD mice had blood in the stool， disease activity index (DAI) and colon tissue myeloperoxidase (MPO) activity were significantly increased (P<0.05)， real-time fluorescent quantitative PCR (Quantitative Real-time PCR) and enzyme-linked immunoassay (ELISA) methods showed that the expression of tumor necrosis factor (TNF-α) inflammatory factors in colon tissue was significantly increased (P<0.05)， indicating that the intestinal tract of HSFD mice is in a mildly inflammatory state. The analysis of the intestinal barrier function showed that inflammatory cell infiltration occurred in the colonic epithelial tissue of HSFD mice， the crypt structure was destroyed， the goblet cells were reduced， the occluding protein (ZO-1)， the occluding protein (Occludin)， and the mucus protein (the gene expression levels of barrier-related proteins such as MUC-2) were significantly reduced (P<0.05). The immunofluorescence results showed that the expression levels of ZO-1 and Occludin in the colon tissue of HSFD mice were significantly reduced (P<0.05). Fluorescein isothiocyanate-labeled dextran (FITC-Dextran) analysis showed that the intestinal permeability of HSFD mice was significantly increased. Conclusion: HSFD can cause lipid metabolism disorders in mice， destroy the intestinal barrier function， and promote the intestinal tract to be in a state of inflammation， which may cause chronic intestinal lesions.