负载白藜芦醇的大麦醇溶蛋白/海藻酸丙二醇酯复合纳米颗粒的制备及表征
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(1.中国农业大学食品科学与营养工程学院 北京 100083;2.农业农村部农业转基因生物安全评价(食用)重点实验室 北京 100083)

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2018年国家科技重大专项RNA干扰新型转基因产品食用安全评价关键技术与评价指南研究(2018ZX0801102B-002)


Fabrication and Characterization of Resveratrol Loaded with Hordein / Propylene Glycol Alginate Composite Nanoparticles
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(1.College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083;2.Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety),Ministry of Agriculture and Rural Affairs, Beijing 100083)

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    摘要:

    以大麦醇溶蛋白(hordein)、海藻酸丙二醇酯(PGA)为原料,通过溶剂蒸发法制备大麦醇溶蛋白-海藻酸丙二醇酯复合纳米颗粒(Hordein/PGA),并对白藜芦醇进行包封。结果表明,随海藻酸丙二醇酯添加量的增加,Hordein/PGA的粒径由1 654.9 nm逐渐下降至475.9 nm。同时Hordein/PGA的电位均为负值,并随海藻酸丙二醇酯添加量的增大由-3.62 mV下降至-8.04 mV。红外光谱结果显示:大麦醇溶蛋白与海藻酸丙二醇酯主要通过氢键、静电吸引及疏水相互作用结合。以Hordein/PGA1∶1对白藜芦醇进行包封,构建三元复合纳米颗粒(Hordein/PGA/Res),白藜芦醇的包封率可达87%。红外光谱和X射线衍射分析表明白藜芦醇与大麦醇溶蛋白、海藻酸丙二醇酯有效结合。Hordein/PGA/Res经85 ℃处理30 min后白藜芦醇保留率为88%,显著高于游离白藜芦醇保留率(73%);经6 h紫外光辐射处理后白藜芦醇保留率可提高约16%。

    Abstract:

    In this study, Hordein/PGA composite nanoparticles were fabricated by solvent evaporation using hordein and PGA as materials, and resveratrol was encapsulated. The results indicated that the particle size of Hordein/PGA decreased from 1 654.9 nm to 475.9 nm with the increase of PGA content. The potentials of Hordein/PGA were negative, and the value decreased from -3.62 mV to -8.04 mV with the increase of PGA content. Fluorescence spectra showed that hydrogen bonds, hydrophobic effects and electrostatic interactions contributed to the formation of Hordein/PGA composite nanoparticles. Resveratrol was encapsulated by Hordein/PGA1:1 to construct ternary composite nanoparticles (Hordein/PGA/Res), and the encapsulation rate of resveratrol reached 87%. Fourier transform infrared and X-ray diffraction demonstrated that resveratrol was effectively combined with hordein and PGA. The retention rate of resveratrol in Hordein/PGA/Res after heat treatment at 85 ℃ for 30 min was 88%, which remarkably higher than the free resveratrol (73%). And the retention rate of resveratrol increased by about 16% after 6 h ultraviolet light treatment.

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赵天瑜,唐荣,金雨楠,梅晓宏.负载白藜芦醇的大麦醇溶蛋白/海藻酸丙二醇酯复合纳米颗粒的制备及表征[J].中国食品学报,2023,23(9):89-98

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  • 收稿日期:2022-09-30
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  • 在线发布日期: 2023-11-22
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