In order to prepare lutein starch nanoparticles (SNPs-lutein)， the starch nanoparticles were prepared by ultrasonic assisted alcohol precipitation method using cassava starch as raw material and lutein alcohol solution as non-solvent phase， and the lutein embedding load was realized simultaneously. When the mass fraction of 3% starch milk， the mass concentration of lutein solution 1 mg/mL， the volume ratio of starch milk to lutein alcohol solution 3 ∶ 10， the embedding amount of lutein reached (57.62±0.36) mg/g and the embedding rate (40.33±0.56)%. Field emission scanning electron microscopy showed that the simultaneous embedding of lutein had no significant effect on the morphology of starch nanoparticles， which were spherical or elliptical particles with smooth surfaces. The SNPs-lutein size distribution was in the 200-600 nm range. Fourier transform infrared spectroscopy showed that the C-H characteristic peak of SNPs-lutein appeared at 2 856 cm-1， and the H-O-H vibration shifted blue at 1 645 cm-1. X-ray diffraction showed that compared with the large envelope peak of SNPs， the V-shaped starch characteristic diffraction peaks of SNPs-lutein appeared at 7.8°，13.1° and 20.9°. The storage stability test showed that the retention rate of lutein in SNPs-lutein was higher than that of lutein raw material at room temperature (25 ℃) and temperature rise (50 ℃) within 30 days.