Based on iTRAQ (isobaric tags for relative and absolute quantity， iTRAQ) technology， the bioinformatics analysis of differential proteomics were carried out with the fruits of ‘Ningqi No. 1’ wolfberry collected from Zhongning， Ningxia as samples and the fruits of ‘Ningqi No. 1’ wolfberry collected from Jinghe， Xinjiang， wulateqian banner， Inner Mongolia， Guazhou， Gansu and Delingha， Qinghai as controls. The results showed that there were 446， 166， 966 and 1 015 differentially expressed proteins (DEPs) in Xinjiang/ Ningxia group， Inner Mongolia/ Ningxia group， Gansu/ Ningxia group and Qinghai/ Ningxia group respectively， and 160 DEPs related to Lycium barbarum polysaccharide synthesis and metabolism. Through further screening， 23 DEPs related to Lycium barbarum polysaccharides were obtained， including sucrose phosphate synthase， β-Glucosidase and α-Galactosidase， etc. It was speculated that the different pathways of polysaccharide anabolism in Lycium barbarum fruits from different producing areas mainly included sucrose， cellulose， galactan， hemicellulose and pectin anabolism， and these processes can be linked together with UDP-glucose. This study analyzed the related proteins in the synthesis pathways of Lycium barbarum polysaccharides by proteomics， which provides a theoretical basis for further study on the regulation mechanism of the same Lycium barbarum polysaccharide from different producing areas.