The process of rapid separation and purification of buckwheat hull flavonoids by zinc ion complexation was studied and the inhibitory activity of purified components on Advanced glycation end products (AGEs) were evaluated. The fraction of intermediates of buckwheat hull flavonoids (IBHF) was obtained by complexing buckwheat hull flavonoids (BHF) through a single zinc ion complexation based on complexation conversion rate. And the other fraction of high purity buckwheat hull flavonoids (HBHF) was obtained through a second zinc ion complexation based on complexation strength. The optimum process parameters of IBHF components obtained by Box-Behnken response surface analysis were zinc acetate to sample mass ratio of 2 ∶ 1， ethanol volume fraction of 30%， ammonia volume fraction of 0.05%， and reaction time of 10 min at room temperature. Under this condition， the complexation conversion was 97.44%. IBHF component were complexed through a second zinc ion complexation to obtain HBHF component. The content of total flavonoids was 57.08 g RE/100 g (aluminum trichloride acetate colorimetry)， and its purity was 6.64 times higher than that before separation and purification. High performance liquid chromatography (HPLC) analysis showed that HBHF component contained isoorientin， vitexin， isovitexin， rutin and kamanophenol-3-O-rutinoside， among which rutin accounted for 62.34%. The AGEs inhibition rate of HBHF was 63.16% in Glu-BSA system and 42.98% in MGO-BSA system at a mass concentration of 200 μg/mL， showing good anti-AGEs activity. Its activity was significantly higher than that of positive control amino guanidine.