荔枝果渣不溶性膳食纤维的结构、体外抗氧化及降血糖活性评价
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(1.广东省农业科学院蚕业与农产品加工研究所 农业农村部功能食品重点实验室广东省农产品加工重点实验室 广州 510610;2.华南农业大学食品学院 广州 510642;3.仲恺农业工程学院轻工食品学院 广州 510631)

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岭南现代农业科学与技术广东省实验室茂名分中心专项(2011ZD008);广东省农业科学院团队建设和人才培养专项(202109TD,R2020PY -JG009)


Evaluation of the Structure, in Vitro Antioxidant and Hypoglycemic Activity of Insoluble Dietary Fiber from Litchi Pomace
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(1.Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs,Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610;2.College of Food Sciences, South China Agricultural University, Guangzhou 510642;3.College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou 510631)

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    摘要:

    为研究不同提取方法对荔枝果渣不溶性膳食纤维(IDF)的理化、结构及功能特性的影响,通过酶解、超声波、高压热水和高静水压4种方法制备荔枝果渣IDF,利用激光粒度仪、高效液相色谱等仪器分析荔枝果渣IDF的粒径、单糖组成等理化结构特性,并探究荔枝果渣IDF体外抗氧化和降血糖能力。结果表明:酶法提取的荔枝果渣IDF平均粒径最小为(11.88±1.19) μm,酶法和超声波法提取的荔枝果渣IDF含有更多的鼠李糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖。各纤维样品均存在典型的纤维素多糖特征吸收峰和纤维素Ⅰ型结构,其微观结构存在差异。功能性质表明,高压热水法提取的荔枝果渣IDF总酚含量高达(13.23±0.32) mg GAE/g,对DPPH和ABTS自由基清除效果最为显著。高压热水法和高静水压法提取的荔枝果渣IDF表现出最强的α-葡萄糖苷酶抑制活性。超声波法提取的荔枝果渣IDF的葡萄糖束缚能力和葡萄糖透析延迟能力均显著高于其它样品。结论:荔枝果渣IDF具有潜在的抗氧化和降血糖活性,可作为功能性产品的良好来源。

    Abstract:

    In order to study the effects of different extraction methods on the physicochemical, structural and functional properties of insoluble dietary fiber (IDF) from litchi pomace, four methods of enzymatic hydrolysis, ultrasound, high-pressure hot water and high hydrostatic pressure were used to prepare IDF from litchi pomace, and the color, particle size, monosaccharide composition and structural properties of IDF from litchi pomace were analyzed by laser particle size meter, high performance liquid chromatography and fourier infrared spectroscopy, and the in vitro antioxidant and hypoglycaemic abilities of litchi pomace IDFs were investigated. The results showed that the average particle size of litchi pomace IDF extracted by the enzymatic method was the smallest [(11.88±1.19) μm]. The enzymatic and ultrasonic methods contained more rhamnose, galacturonic acid, glucose, galactose and arabinose. The structural properties showed no significant changes in the characteristic absorption peaks and crystal conformation of the cellulose polysaccharides for each fiber sample, while there were differences in the microstructure. The surface structure of each litchi pomace IDF was rough and loose, with many folds and gaps. Characteristically, the surface morphology of the litchi pomace IDF extracted by the ultrasonic method was puffy, with a high number of fibrous bundles present. The functional properties showed that the total phenolic content of litchi pomace IDF extracted by high-pressure hot water method was up to (13.23±0.32) mg GAE/g, with the most significant effect on the scavenging of DPPH and ABTS radicals. The litchi pomace IDF extracted by high-pressure hot water method and high hydrostatic pressure method showed the strongest α-glucosidase inhibitory activity. The glucose binding ability and the delayed glucose dialysis capacity of the ultrasonic extracted litchi pomace IDF were significantly higher than those of the other samples. In conclusion, litchi pomace IDF has potential antioxidant and hypoglycemic activities and can be a good resource for functional product.

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李依娜,余元善,胡腾根,李璐,吴继军,肖更生,邹波,卜智斌.荔枝果渣不溶性膳食纤维的结构、体外抗氧化及降血糖活性评价[J].中国食品学报,2023,23(10):195-205

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  • 收稿日期:2022-10-22
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  • 在线发布日期: 2023-12-04
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