In order to obtain waterborne polyurethane (WPU) coatings with excellent inhibition properties against Shewanella putrefaciens bacteria biofilm in aquatic products， SiOx/WPU coatings were prepared by drop coating method with SiOx nanoparticles of different micromorphology as roughness construction factors. The hydrophobicity and oleophobic， surface energy， surface morphology and thermal stability of the modified SiOx/WPU coatings were measured. The inhibition mechanism of the modified SiOx/WPU coatings on the Shewanella putrefaciens biofilm was revealed. The results show that， using SiOx particles modified by cetyltrimethylammonium bromide (CTAB) as the roughness construction factor， the C-SiOx/WPU coating surface has the highest fluorine content， but its contact angles of water and n-hexadecane are 139.0°±3.5° and 0°， respectively， and shows hydrophobic and super-lipophilic， prepared by using SiOx particles modified by and has the worst thermal stability and anti-bacterial adhesion. The modified fumed nano-SiOx /WPU coating is super-hydrophobic and super-lipophilic， which has the highest thermal stability and could inhibit the initial adhesion of Shewanella putrefaciens. The modified micron scale SiOx/WPU coating was prepared by improved St?觟ber method and modified SiOx micro-nano particles in the same solvent system as the roughness construction factor. The modified micron scale SiOx/WPU coating was super-amphiphobic surface with high thermal stability， which could effectively inhibit the irreversible adhesion of Shewanella putrefaciens， reduce its metabolic activity and EPS secretion， and microcolonies just began to form on its surface， after 24 h of culture. The super-amphiphobic modified micron scale SiOx/WPU coatings in this study can be used to prepare food packaging materials， providing technical support for the application of anti-biofilm materials in the field of food packaging.