Short chain-galactooligosaccharides (SC-GOS) is an effective proliferation factor of Bifidobacteria. Seven strains of Bifidobacteria with high utilization of SC-GOS were screened from infant fecal samples， the result of 16S rDNA identification was that one was Bifidobacterium longum subsp. infantis BI_Y46， 2 B. pseudocatenulatum (BP_Y43， BP_YA)， 2 B. bifidum (BB_Y22， BB_S7) and 2 strains of B. longum (BL_S34， BL_H26). Compared with B. infantis M63 and B. infantis ATCC 15697， the morphology of the strains was observed and the probiotic characteristics were analyzed. Thus， the probiotics with application potential were screened. The results showed that BI_Y46 had special morphology with bifurcated fimbriae， and the exopolysaccharide yield of BB_Y22 and BP_Y43 was higher than 0.45 mg/mL. The surface protein content of BL_H26 was (0.74±0.07) mg/mL， the content of BB_S7 and BB_Y22 was between 0.35-0.45 mg/mL. Whereas， BP_Y43 and BB_Y22 had strong surface hydrophobicity and self-aggregation ability. BB_Y22 and BI_Y46 showed strong tolerance to the stimulation of bile salt and gastric juice. The supernatant of BI_Y46 and BB_S7 had strong inhibiting effect on Escherichia coli ATCC 25922. In summary， B. infantis BI_Y46， B. bifidum BB_Y22 and B. pseudocatenulatum BP_Y43 had good probiotic potential and provide some reference for the development of products containing galactoseoligosaccharides and Bifidobacterium.