HS-GC-IMS结合化学计量学分析6种野生蜂蜜的挥发性风味成分
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(1.云南农业大学食品科学技术学院 昆明 650201;2.食药同源资源开发与利用教育部工程研究中心 昆明 650201;3.云南省药食同源功能食品工程研究中心 昆明 650201)

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云南省重大科技专项计划(202002AA100005,202102AE090027-2);云南省万人计划产业技术领军人才项目(YNWR-CYJS-2020-010)


Analysis of Volatile Flavor Components of Six Wild Honeys Based on HS-GC-IMS Combined with Chemometrics
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(1.College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201;2.Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education, Kunming 650201;3.Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food,Kunming 650201)

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    摘要:

    为明确云南6种主要野生蜂蜜的挥发性物质种类以及区分它们之间挥发性化合物成分的差异,采用顶空气相离子迁移谱技术(HS-GC-IMS)对江城野生大挂蜜、野生黑蜜、野生崖蜜、野生小挂蜜、野生树洞蜜和澜沧野生大挂蜜的特征风味成分进行分析。通过LAV软件构建不同品种蜂蜜挥发性风味指纹图谱,比较云南省主要品种野生蜂蜜的特征风味物质差异,确定其特征风味化合物及相对含量。结果表明,6种蜜蜂样品所含的风味物质均具有一定差异,江城野生大挂蜜和野生小挂蜜差异较大,野生黑蜜和野生树洞蜜差异次之,野生崖蜜、野生小挂蜜、野生树洞蜜和澜沧野生大挂蜜相似度较高。共检出80种挥发性物质,定性检出65种,包括醛类24种、醇类22种、酮类6种、酯类7种、呋喃3种、吡嗪类1种、酸类1种、醚类1种。筛选出20种特征挥发性标志物(VIP>1),包括1-庚醇、6-甲基-5-庚烯-2-酮、2-甲基丙酸乙酯、乙酸乙酯、己醛、环己酮、芳樟醇、丁醛、二丙基二硫醚、香茅醇、(E)-2-己烯醛、氧化芳樟醇、2-己醇、2-戊基呋喃、1-庚醇、(E)-2-庚烯醛、苯乙醛、苯甲醇、异丁醇和2-呋喃甲醇。主成分分析和聚类分析显示不同品种的蜂蜜得到明显区分,表明HS-GC-IMS技术可用于野生蜂蜜的鉴别。

    Abstract:

    To distinguish the differences in volatile species and components of six wild honeys in Yunnan, headspace-gas chromatography-ion mobility spectroscopy(HS-GC-IMS) was used to analyze the characteristic flavor components of jiangcheng wild big dagua honey, wild black honey, wild cliff honey, wild small dagua honey, wild tree cave honey and lancang wild big dagua Honey. The volatile flavor fingerprints of different varieties of honey were constructed by LAV software to compare the differences in the characteristic flavor substances of different varieties of wild honey in Yunnan province and to determine the main characteristic flavor compounds and their relative contents. The results showed that there were some differences in the flavor substances of the six kinds of bees. The difference between jiangcheng wild dagua honey and wild xiaogua honey was the largest, followed by the difference between wild black honey and wild tree hole honey, and the similarity between wild cliff honey, wild wild xiaogua honey, wild tree hole honey and lancang wild dagua honey was higher. A total of 80 volatile substances were detected and 65 were qualitatively detected, including 24 aldehydes, 22 alcohols, 6 ketones, 7 esters, 3 furans, 1 pyrazine, and 1 hydrocarbon , 1 type of acids, 1 type of ethers. Twenty characteristic volatile markers (VIP>1) were screened by partial least squares-discriminant analysis, including 1-heptanol, 6-methyl-5-hepten-2-one, ethyl 2-methylpropionate, ethyl acetate, hexanal, cyclohexanone, linalool, butyraldehyde, dipropyl disulfide, citronellol, (E)-2-hexenal, oxidized linalool, 2-hexanol, 2-pentylfuran, 1-heptanol, (E)-2-heptenal, phenylacetaldehyde, benzyl alcohol, isobutanol, and 2-furanyl alcohol. Principal component analysis and cluster analysis showed that different varieties of honey were clearly distinguished, indicating that the HS-GC-IMS technique can be used to distinguish honey.

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顾凡,陶亮,代佳和,李昌源,汤木果,田洋. HS-GC-IMS结合化学计量学分析6种野生蜂蜜的挥发性风味成分[J].中国食品学报,2023,23(11):320-331

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  • 收稿日期:2022-11-10
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  • 在线发布日期: 2023-12-14
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