In order to efficiently and rapidly detect Salmonella in milk， and improve the sensitivity of the anti-fouling biosensor. In this study， amphoteric poly (sulfobetaine methacrylate) (SBMA)-modified swab was used to obtain the anti-fouling property， and constructed a sensing platform with 'blue latex microspheres/adaptor' as the colorimetric signal probe for the rapid detection of Salmonella in milk samples. The linear detection of Salmonella was 103-107 CFU/mL， and the detection limit was 117.80 CFU/mL. The detection is completed in 20 min， which can be qualitative to the naked eye， can be combined with the detection of portable colorimeter smartphones， can be measured and read， and is easy to operate， which is also a rapid detection of Salmonella.