Objective: To investigate the effects of preservative (dehydroacetic acid and ε-polylysine hydrochloride) and incubation temperature on the growth and toxin production of Burkholderia gladiolus on wet rice flour medium. Methods: A Burkholderia acorus(P. coenovenae) strain isolated from a food poisoning event was used as the research object. The strains were inoculated into wet rice flour medium supplemented with dehydroacetic acid (1.0 g/kg)， ε-polylysine hydrochloride(0.25 g/kg) and no preservative， and cultured at 10， 26 and 36 ℃ to study the growth and virulence of the strains in wet rice flour. The modified Gompertz model was used to construct the primary growth model. Results: At 10 ℃， the strain grew slowly and did not produce bongkrekic acid. At 26 ℃， the strain produced toxin more than 500 μg/kg in the control group and all experimental groups when cultured to 96 h. The strain produced bongkrekic acid the earliest in the samples with dehydroacetic acid， and its maximum concentration (1 484 μg/kg) was slightly lower than that in the control group (2 561 μg/kg) and ε-polylysine hydrochloride group (2 762 μg/kg). The most rapid growth was observed at 36 ℃， but the production of toxin was lower than 350 μg/kg. Conclusion: Storage of wet rice flour at 10 ℃ can effectively reduce the risk of producing rice krekic acid. Both dehydroacetic acid and ε-polylysine hydrochloride failed to prevent the growth and toxin production of Burkholderia gladiolus. It is necessary to further explore effective preservants to develop multi-means control measures.