In order to clarify the application potential and application scope of crude powder in food processing and storage， the antibacterial spectrum was first determined. Using Candida albicans as the indicator bacteria， the relative antibacterial activity of crude powder was evaluated from the minimum inhibitory concentration， temperature， pH， salt concentration，repeated freezing and storage time， and finally the safety evaluation was conducted. The results showed that， crude powder of Lactobacillus-derived antimicrobial peptide has broad-spectrum antibacterial properties； With its good thermal stability， 121 ℃ still maintain 79.68% relative antibacterial activity for 20 min； pH 2-6 treatment maintained over 90% of the relative antibacterial activity after 4 h， almost complete inactivation after 4 h of pH 10-12 treatment； When the salt mass concentration reaches 0.8 mg/mL， the relative antibacterial activity was significantly reduced (P＜0.05)； The relative antibacterial activity had no significant change after storage at different low temperatures for different time (P > 0.05)； The relative antibacterial activity after repeated freezing and thawing 5 times at -20 ℃ was also not different (P＞0.05)； The safety evaluation confirmed that crude powder belongs to the actual non-toxic safety level. In conclusion， it can be seen that the crude powder of Lactobacillus-derived antimicrobial peptide has good stability and a wide range of applications， and has broad application prospects as a biological preservative in the food field.