In order to explore the changes of blueberry quality after microenvironment fluctuations (i.e.， temperature fluctuations， gas environment fluctuations， and temperature and gas fluctuations) under the state of ice temperature and gas regulation， Lanfeng blueberry was taken as the test material and pre-cooled at different ice temperature (-2 ℃ and -1 ℃) for air conditioning (CO₂: 10%-14%； O₂: 6%-9%) were stored in the environment for 7 days， and the optimal environmental state of blueberry fruit was analyzed. On this basis， three groups of microenvironment fluctuation tests were designed， namely， temperature fluctuation， air fluctuation， temperature and air fluctuation， which fluctuated for 1aq to 6 days， respectively. After each fluctuation， corresponding measures were taken for storage. The hardness， cohesion， titrable acid content， anthocyanin content and malonaldehyde content of blueberry fruits in each experimental group were measured for 1 to 7 days. The results showed that the fresh-keeping effect of experimental group was better. In the microenvironment fluctuation test， the optimal storage time of blueberry was 1 day. The optimal ice temperature and air conditioning environment of Lanfeng blueberry fruit was -1 ℃ (CO₂:10%-12%， O₂:6%-9%)， and there was a negative correlation between blueberry fruit quality and fluctuation time. Although cooling/oxygen lowering/double descending methods had certain effects， the blueberry fruit quality could not be restored to the best state in the ice temperature and air conditioning group.