乌衣红曲黄酒年份特征挥发性物质指纹图谱分析
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(1.福建省农业科学院农产品加工研究所 福州 350003;2.农业农村部亚热带特色果蔬菌加工重点实验室 福州 350003;3.福建省农产品(食品)加工重点实验室 福州 350003)

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福建省属公益类科研院所基本科研专项(2020R1032008,2021R1032001)


Fingerprint Analysis of Volatile Substances in the Vintage Characteristics of Wuyi Hongqu Huangjiu
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(1.Institute of Food Science and Technology, Fujian Academy of Agricultural Science, Fuzhou 350003;2.Key Laboratory of Subtropical Characteristic Fruits, Vegetables and Edible Fungi Processing (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Fuzhou 350003;3.Fujian Key Laboratory of Agricultural Products (Food) Processing, Fujian Fuzhou 350003)

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    摘要:

    目的:建立乌衣红曲黄酒基酒挥发性成分指纹图谱,用于年份基酒酒龄的判别。方法:通过顶空固相微萃取-气相色谱联用质谱法(HS-SPME-GC-MS)分析不同陈酿时间的乌衣红曲黄酒中的挥发性成分,结合正交偏最小二乘法-判别分析(OPLS-DA)和Spearman相关性分析确定与年份相关的关键成分,用于构建年份基酒特征指纹图谱。结果:从不同年份的乌衣红曲黄酒中,共检出并定性挥发性成分122种,与陈酿时间显著相关的有20种(|r|>0.5),其中N-苯甲酰甘氨酸乙酯、6-庚烯酸乙酯、乙酸-2-乙基苯酯等8种与陈酿时间呈正相关关系,2-呋喃甲醇、辛酸、庚酸乙酯等12种与陈酿时间呈负相关关系。基于筛选的20种年份特征挥发性物质,构建了乌衣红曲黄酒基酒年份基酒指纹图谱。依据香气成分的OPLS-DA分析和指纹图谱数据信息将2~15年陈乌衣红曲基酒样品区分为青陈酒(2~4年)、中陈酒(5~9年)、老陈酒(10~15年)3个类别,并通过二次取样分析,验证所建图谱的可靠性。结论:GC-MS技术构建的挥发性成分指纹图谱可为乌衣红曲黄酒基酒品质评价及酒龄的识别提供基础数据。

    Abstract:

    Objective: The purpose of this study was to establish the fingerprint of volatile components of Wuyi Hongqu Huangjiu base wine, which could be used to distinguish the age of base wine. Methods: The volatile components in Wuyi Hongqu Huangjiu with different aging time were analyzed by headspace solid phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS), and the key components related to the year were determined by orthogonal partial least squares discriminant analysis (OPLS-DA) and Spearman correlation analysis, which were used to construct the characteristic fingerprint of the year base wine. Results: A total of 122 volatile components were detected and identified from Wuyi Hongqu Huangjiu in different years, and 20 were significantly correlated with aging time (r>0.5 or r < -0.5). Among them, N-benzoyl glycine ethyl ester, 6-heptanoic acid ethyl ester, acetic acid-2-ethyl phenyl ester, 3- (2-furanyl) - 2-acrolein, ethyl phenylacetate were significantly positively correlated with aging time, 2-furan methanol, caprylic acid Ethyl heptanate and other 12 species were negatively correlated with the aging time of Wuyi Hongqu Huangjiu. Based on the selected 20 year characteristic volatile substances, the fingerprint of Wuyi Hongqu Huangjiu base wine was constructed. According to the OPLS-DA analysis of aroma components and the fingerprint data information, the samples of Wuyi Hongqu base wine from 2 to 15 years were divided into three categories: Green wine (2-4 years), medium wine (5-9 years) and old wine (10-15 years), The reliability of the established map was verified by secondary sampling analysis. Conclusion: The fingerprint of volatile components constructed by GC-MS technology could provide basic data support for the quality evaluation and identification of wine age of Wuyi Hongqu Huangjiu.

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苏昊,李维新,林晓姿,何志刚,梁璋成,任香芸.乌衣红曲黄酒年份特征挥发性物质指纹图谱分析[J].中国食品学报,2024,24(2):260-268

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  • 收稿日期:2023-02-20
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  • 在线发布日期: 2024-03-12
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