As a common antibiotic， tetracycline(TC) is extensively used in the prevention and treatment bacterial diseases in animals and is also used as a feed additive to promote the growth of animals. In this paper， a fluorescence quenching method based on nitrogen doped carbon quantum dots(N-CQDs) derived from pineapple peel was established for the rapid detection of TC. The blue fluorescent N-CQDs with high quantum yield were prepared by the hydrothermal method using pineapple peel and diethylenetriamine as precursors. The structure of N-CQDs was characterized by transmission electron microscope， fourier infrared spectroscopy and X-ray photoelectron spectroscopy. The results showed that the average particle size of N-CQDs was about 3.53 nm， which was mainly composed of C， N and O， and the surface has oxygen-containing and nitrogen-containing groups. The fluorescence of N-CQDs was effectively quenched by static quenching and internal filtering effect. Under the optimal experimental conditions， the method had good linearity within 0.3-40 μg/mL， and the detection limit was calculated to be 89.26 μg/L. Moreover， the detection method has been applied to the determination of TC in pork， the recovery rate was 98.40%-106.02%. The results showed that the detection method could be applied to the rapid determination of TC in pork.