Objective: To investigate the effect of sea cucumber cooking liquid (SCCL) dry powder on chronic constipation in mice. Methods: Loperamide hydrochloride was administered intragastrically to establish the chronic constipation model. To evaluate the positive effect of SCCL in intestinal motility of constipation mice， the defecation situation， intestinal ink transit rate and mRNA expression levels of intestinal motility related neurotransmitters and receptors were detected. Result: Compared with the model control group， the intestine ink transit rate of mice in the high-dose SCCL group was significantly increased (P<0.05)， the time of first black faeces was significantly shortened (P<0.01)， and the number and weight of faeces in 6 hours in the low-dose and high-dose SCCL groups were significantly increased (P<0.05)； The number of goblet cells and the content of mucin in the colon of mice were improved significantly (P<0.05)； The mRNA expression levels of M2AChR， M3AChR， 5-HT3R， 5-HT4R and C-Kit in colon tissue were significantly increased (P<0.05). Conclusion: SCCL alleviated constipation by regulating intestinal peristalsis related neurotransmitters such as ACh， 5-HT and its downstream signaling pathways， eventually increased the number of ICC cells， improved intestinal motility and promoted intestinal peristalsis.