UPLC-Q-TOF-MS/MS was used to analyze the metabolite profiles of JFP-Ps (Polysaccharides from Artocarpus heterophyllus Lam. pulp) in the serum of DSS colitis rats. Then metabonomics analysis was used to explore the serum metabolite profiles and metabolic pathway. The results showed that JFP-Ps mainly regulated these metabolites such as amino acids， peptides and their analogues， purines and pyrimidines and their derivatives， lipids and lipid-like molecules. There are 5 metabolic pathways related to enteritis in rat serum， namely arginine biosynthesis， D-arginine and D-ornithine metabolism， phenylalanine， tyrosine and tryptophan biosynthesis， phenylalanine metabolism， arginine and proline metabolism. The results indicate that JFP-Ps can attenuate inflammation by regulating amino acid metabolism and influencing related pathways in rats with DSS-induced colitis.