Objective: Protein fiber films loaded with tea polyphenols were obtained as the active packaging films for tuna， and the characteristics and preservation properties of the packaging film were explored. Method: Zein/ gelatin (ZG) fiber films loaded with different concentration core materials of tea polyphenols (10， 20， 30 mg/mL) was prepared by coaxial electrospun technology. The morphology and characteristics of the fiber film were analyzed by scanning electron microscopy， infrared spectroscopy， X-ray diffraction， differential scanning calorimetry， hydrophilicity and hydrophobicity， antibacterial and antioxidant tests， and preservation properties for tuna was analyzed. Results: The average fiber diameters of ZG， TP1/ZG， TP2/ZG and TP3/ZG films were (810±240)， (930±270)， (880±150) and (850±610) nm， respectively. TP in TP/ZG coaxial nanofibers did not significantly change the chemical structure and crystal structure of the ZG film. Each TP/ZG film has a ZG-like heat absorption peak near less than 130 ℃. The 60 s contact angles of TP1/ZG， TP2/ZG and TP3/ZG are (105.89±5.14)°， (91.32±5.11)° and (73.36±4.20)°， respectively. The thermal properties and water contact angles of TP/ZG films decreased with increasing TP concentration. TP endowed TP/ZG membrane with good antibacterial and antioxidant properties， and has a significant inhibitory effect on Shewanella putrefaciens in aquatic products. TP/ZG can effectively inhibit the total viable counts， pH value， volatile base nitrogen， fat oxidation and texture deterioration of tuna fillets stored at 4 ℃， and can prolong the shelf life of tuna for 3 days. Conclusion: ZG coaxial fiber film loaded with appropriate TP possessed good morphology， water resistance， antibacterial， antioxidant and preservation properties， which can be used as a new type of antibacterial and antioxidant food packaging material.