大黄鱼优势腐败菌的致腐能力及对挥发性风味的影响
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(1.浙江工商大学食品与生物工程学院 杭州 310018;2.浙江工商大学东海食品台州研究院 杭州 310018;3.深圳市市场监督管理局许可审查中心 广东深圳 518000;4.浙江兴旺水产集团有限公司 浙江台州 317000)

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浙江省教育厅一般项目(Y201942135);浙江省自然科学基金项目(LQ21C200004)


The Spoilage Ability of Dominant Spoilage Bacteria in Large Yellow Croaker and Their Impact on Volatile Flavor
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(1.College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018;2.Donghai Food Research Institute (Taizhou), Zhejiang Gongshang University, Hangzhou 310018;3.License Review Center of Shenzhen Municipal Administration for Market Regulation, Shenzhen 518000, Guangdong;4.Zhejiang Xingwang Aquatic Products Group Co., Ltd., Taizhou 317000, Zhejiang)

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    摘要:

    为评估冷藏大黄鱼优势腐败菌的致腐能力,从贮藏末期的鱼肉中分离出7种腐败菌,分析接种7种腐败菌的鱼肉样品的菌落总数值(TVC)、总挥发性盐基氮值(TNB-N)和挥发性成分组成。结果表明,接种不同菌株的鱼肉样品间的TVC值呈现显著性差异(P<0.05),其中魏氏假单胞菌、波罗的海希瓦氏菌、麦芽糖肉食杆菌在贮藏末期增长缓慢;接种液化沙雷氏菌、软体气单胞菌、波罗的海希瓦氏菌的TNB-N值增幅较快,与初始时相比分别增加了7.31%,4.92%,7.03%,而对照组仅增加0.98%。产量因子结果显示:波罗的海希瓦氏菌是真空包装大黄鱼贮藏期间的优势腐败菌,其末期值高达46.58 × 10-8 mg TVB-N/CFU,其次是液化沙雷氏菌和软体气单胞菌。通过气相色谱-离子迁移谱(GC-IMS)共鉴定出81种挥发性风味物质,其中对照组主要产生醚类、醇类、烯类、噻唑类物质。明显不同于对照组,在7种腐败菌作用下挥发性成分主要为酯类、醛酮类、烃类以及含氮含硫类化合物,接种不同菌种后鱼块的气味物质种类存在着较大的差异,不同接菌鱼块会产生其特有的风味物质。本研究为后期进一步探究贮藏大黄鱼的腐败机制提供参考。

    Abstract:

    To evaluate the putrefactive potential of dominant putrefactive bacteria in refrigerated large yellow croaker, seven putrefactive bacteria were isolated from fish meat at the end of storage, and analyzed the total bacterial count (TVC), total volatile base nitrogen value (TNB-N) and volatile component composition of fish meat samples inoculated with 7 types of spoilage bacteria. The results showed that there was a significant difference in the TVC values among the fish samples inoculated with different strains (P<0.05). Among them, Pseudomonas weihenstephanensis, Shewanella baltica strain and Carnobacterium maltaromaticum grew slowly at the end of storage; The TNB-N values increased rapidly after inoculation with Serratia liquefaciens, Aeromonas molluscorum and Shewanella baltica, with increases of 7.31%, 4.92%, and 7.03% compared to the initial stage, respectively, while the control group only increased by 0.98%; The yield factor results showed that Shewanella baltica was the dominant spoilage bacterium during the storage period of vacuum packaged large yellow croaker, with a final value of 46.58 × 10-8 mg TVB-N/CFU, followed by Serratia liquefaciens, Aeromonas molluscorum; A total of 81 volatile flavor compounds were identified by gas chromatography-ion migration spectrometry (GC-IMS), with the control group mainly producing ether, alcohol, ene, and thiazole compounds. Obviously different from the control group, The volatile components under the action of 7 types of spoilage bacteria were mainly esters, aldehydes and ketones, hydrocarbons, and nitrogen and sulfur compounds. After inoculation with different bacterial strains, the types of odor compounds in fish pieces showed significant differences and different inoculated fish pieces produced their unique flavor compounds. This study provides a reference for further exploring the spoilage mechanism of stored yellow croaker in the future.

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柴庭庭,朱星橦,柴保臣,陈军辉,陈跃文.大黄鱼优势腐败菌的致腐能力及对挥发性风味的影响[J].中国食品学报,2024,24(6):329-340

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  • 收稿日期:2023-09-15
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  • 在线发布日期: 2024-07-22
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