干酪乳杆菌发酵豆粉粗多糖结构特征与抗氧化活性
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(1.江汉大学生命科学学院 品营养与安全研究中心 武汉 430056;2.湖北省豆类(蔬菜)植物工程技术研究中心 武汉 430056)

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市属高校产学研项目(CXY202202);湖北省高价值知识产权培育工程项目(鄂知发[2021]2号);湖北省重点研发计划项目(2022BBA0064);教育部产学合作协同育人项目(231007156305207)


Structural Characteristics and Antioxidant Activities of Crude Polysaccharides from Fermented Bean Flour by Lactobacillus casei
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(1.Research Center of Food Nutrition and Safety, School of Life Sciences, Jianghan University, Wuhan 430056;2.Hubei Province Engineering Research Center for Legume Plants, Wuhan 430056)

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    摘要:

    制备8种干酪乳杆菌发酵豆粉粗多糖样品,比较其粗多糖得率、结构特征和抗氧化活性的差异。结果表明:发酵豆粉粗多糖得率为1.07%~11.42%,其中发酵红豆粉粗多糖得率最高(11.42%)。发酵豆粉粗多糖中均含有糖醛酸结构,其中发酵菜豆粉粗多糖中糖醛酸含量最高(7.46%)。发酵豆粉粗多糖分子质量为0.35~36.67 ku,其中发酵绿豆粉粗多糖含有的多糖分子种类最多。发酵豆粉粗多糖由甘露糖、葡萄糖醛酸、葡萄糖、半乳糖、木糖和阿拉伯糖以不同含量组成。发酵豆粉粗多糖表观形态呈片状松散状态且形状不规则,其中发酵绿豆粉粗多糖表现出最疏松程度。红外光谱显示8种发酵豆粉粗多糖均符合多糖化合物的特征吸收。8种发酵豆粉粗多糖表现出不同的抗氧化活性:发酵扁豆粉和发酵绿豆粉粗多糖清除DPPH的IC50值分别为0.07 mg/mL和0.98 mg/mL,发酵扁豆粉粗多糖清除效果最好;发酵绿豆粉粗多糖清除ABTS+的能力最好,其IC50值为0.67 mg/mL;发酵绿豆粉粗多糖对铁离子也表现出最高的还原能力。比较8种发酵豆粉粗多糖,发酵绿豆粉粗多糖表现出最佳的抗氧化活性。研究结果对干酪乳杆菌发酵制备不同豆粉食品的营养评价提供了理论依据。

    Abstract:

    In this study, the samples of crude polysaccharides were prepared from 8 kinds of fermented bean flour by Lactobacillus casei, and the yield, structural characteristics and antioxidant activities of these crude polysaccharides were compared and analyzed. The results showed that the yields of crude polysaccharides were between 1.07% and 11.42%, and the yield of crude polysaccharides from fermented red bean flour showed the highest value (11.42%). All of the crude polysaccharides contained uronic acid, and the uronic acid in crude polysaccharides from fermented kidney bean flour showed the highest content 7.46%. The molecular weight of main polysaccharides was between 0.35-36.67 ku, and the crude polysaccharides from fermented mung bean flour contained the most types of polysaccharide molecules. The crude polysaccharides were composed of mannose, glucuronic acid, glucose, galactose, xylose and arabinose with different contents. The apparent form of crude polysaccharides was flaky, loose and irregular, and the crude polysaccharide from fermented mung bean flour showed the highest degree of looseness. The infrared spectra of crude polysaccharides from 8 kinds of fermented bean flour was consistent with the characteristic absorption of polysaccharide compounds. The crude polysaccharides from 8 kinds of fermented bean flour showed different antioxidant activities: the IC50 values for scavenging DPPH radical of fermented lentil and crude mung bean polysaccharides were 0.07 mg/mL and 0.98 mg/mL, respectively, the crude polysaccharides from fermented lentil flour had the best scavenging effect on DPPH radical. The IC50 value for scavenging ABTS radical cation of crude polysaccharides from fermented mung bean flour was 0.67 mg/mL, which showed the best scavenging ability for ABTS radical cation and crude polysaccharides from mung bean flour also showed the highest reduction ability to iron ions. Compared with the crude polysaccharides from 8 kinds of fermented bean flour by Lactobacillus casei, the crude polysaccharides from fermented mung bean flour showed the best antioxidant activity. It provided an important theoretical basis for the nutritional evaluation of fermented bean foods by Lactobacillus casei.

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屈雅宁,许梦粤,李慧,王璐,郭瑞,王红波.干酪乳杆菌发酵豆粉粗多糖结构特征与抗氧化活性[J].中国食品学报,2024,24(8):112-121

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  • 收稿日期:2023-08-24
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  • 在线发布日期: 2024-09-26
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