卡拉胶/速溶琼胶包埋体系负载茶多酚的特性
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(1.集美大学海洋食品与生物工程学院 福建厦门 361021;2.厦门海洋职业技术学院 福建厦门 361100;3.福建省食品微生物与酶工程重点实验室 福建厦门 361021;4.海洋食品精深加工关键技术省部共建协同创新中心 大连工业大学 辽宁大连 116034)

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国家自然科学基金青年科学基金项目(32001713);福建省科技重大专项专题项目(2020NZ012015);厦门市海洋与渔业发展专项资金项目(21CZP 005HJ07)


Characterization of Carrageenan/ Instant Agar Embedding Systems Loaded with Tea Polyphenols
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(1.College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, Fujian;2.Xiamen Ocean Vocational College, Xiamen 361100, Fujian;3.Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, Fujian;4.Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning)

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    摘要:

    为提高茶多酚的稳定性和生物利用率,以卡拉胶和速溶琼胶分别复合乳清蛋白为壁材,制备茶多酚的包埋负载体系。以茶多酚包埋率为主要优化指标,考察卡拉胶和速溶琼胶复合乳清蛋白对茶多酚的负载效果。结果表明:卡拉胶-乳清蛋白包埋体系在pH 2、芯壁比1∶20、壁材质量分数6%时,对茶多酚的负载效果最优,包埋率为(91.94±0.31)%。速溶琼胶-乳清蛋白包埋体系在pH 4、芯壁比1∶25、壁材质量分数为4%时,包埋率最高,达到(98.48±0.39)%。抗氧化活性测定结果表明,卡拉胶和速溶琼胶复合体系的DPPH自由基清除率分别高至(49.55±0.00)%和(48.35±0.52)%,两者ABTS自由基清除率最高达到(90.90±0.23)%和(81.35±0.90)%,具有良好的抗氧化性能。说明卡拉胶-乳清蛋白包埋体系和速溶琼胶-乳清蛋白包埋体系均能够实现对茶多酚的有效保护,尤其是速溶琼胶体系。研究成果可为海藻胶体作为活性成分的递送壁材提供设计思路。

    Abstract:

    In order to improve the stability and bioavailability of tea polyphenols(TP), this paper was to prepare the embedding system of tea polyphenols, which use carrageenan or instant agar compounded with whey protein as wall materials. The embedding rate of tea polyphenol was selected as the main optimization index to investigate the embedding effect of carrageenan or instant agar compounded with whey protein on tea polyphenols. The results showed the highest embedding rate of (91.94±0.31)% when the carrageenan-whey protein embedding system prepared at pH 2, core-to-wall ratio of 1∶20 and wall mass fraction of 6%. While the instant agar-whey protein embedding system had the maximum embedding rate of (98.48±0.39)% when prepared at pH 4, core-to-wall ratio of 1∶25 and wall mass fraction of 4%. The results of the antioxidant activity measurements suggested that both carrageenan and instant agar complex systems with good antioxidant properties, where the DPPH radical scavenging rate of the carrageenan and instant agar systems was as high as (49.55±0.00)% and (48.35±0.52)%, respectively, and the highest ABTS radical scavenging rates of (90.90± 0.23)% and (81.35±0.90)%. Both the carrageenan-whey protein embedding system and the instant agar-whey protein embedding system can effectively protect the tea polyphenols, especially for instant agar system can improve the storage stability of tea polyphenols. Our findings can help the design and development of seaweed hydrocolloids as embedding wall for bioactive ingredient.

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卢奕如,倪辉,朱艳冰,姜泽东,李清彪,郑明静.卡拉胶/速溶琼胶包埋体系负载茶多酚的特性[J].中国食品学报,2024,24(9):40-50

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  • 收稿日期:2023-09-26
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  • 在线发布日期: 2024-10-21
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