泡菜中产β-葡萄糖苷酶植物乳杆菌的益生和酶学特性
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(1.渤海大学食品科学与工程学院 辽宁锦州 121013;2.杭州萧山农业发展有限公司 杭州 311215)

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Probiotic and Enzymatic Characteristics of β-glucosidase Producing Lactobacillus plantarum Isolated from Pickled Vegetables
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(1.College of Food Science and Engineering, Bohai University, Jinzhou 121013, Liaoning;2.Hangzhou Xiaoshan Agriculture Development Co., Ltd., Hangzhou 311215)

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    摘要:

    乳酸菌因自身特性和分泌多种代谢物而在发酵过程中起重要的作用。从泡菜中筛选出2株高产β-葡萄糖苷酶乳酸菌,经16S rRNA基因测序比对后均鉴定为植物乳杆菌(命名为PC7-C6和PC7-C11)。随后对2菌株的益生特性以及酶产量较高的PC7-C11的酶学特性进行分析。结果显示:2菌株为γ-溶血,对氨苄西林、克拉霉素敏感,对四环素、红霉素和克林霉素中度敏感;可在pH 4条件下生长;培养8 h后自聚集率在50%~64%范围,与大肠杆菌共聚集率均大于60%;对乙酸乙酯的疏水率分别为70.41%,61.19%,对二甲苯和正己烷的疏水率在30%~40%范围;能够抑制大肠杆菌、蜂房哈夫尼菌、腐生葡萄球菌、粘质沙雷氏菌和马胃葡萄球菌等细菌的生长;PC7-C11的抗氧化能力优于PC7-C6,PC7-C11对DPPH自由基清除率和羟自由基清除率分别为59.33%和53.49%。植物乳杆菌PC7-C11菌株酶活力的最适反应温度40 ℃、最适pH值7,乙醇、葡萄糖、二甲基亚砜、SDS、EDTA、Fe2+、Mg2+、Na+、Ca2+会不同程度地抑制酶活,K+对酶活力有一定的促进作用。

    Abstract:

    Lactic acid bacteria play an essential role in fermentation due to their characteristics and secretion of various metabolites. In this study, two lactic acid bacteria with high β-glucosidase activities were screened from kimchi and identified as Lactobacillus plantarum PC7-C6 and PC7-C11). The probiotic characteristics of the two strains and the enzymatic characteristics of PC7-C11which has higher enzyme production ability, were analyzed. The results showed that the two strains were γ-hemolysis, sensitive to ampicillin and clarithromycin, moderately sensitive to tetracycline, erythromycin, and clindamycin, and could grew at pH 4. The auto-aggregation rate of the two isolates ranged from 50% to 64%, and the co-aggregation rates with Escherichia coli were more than 60% after co-incubating for 8 hours. The hydrophobicity of the isolates towards ethyl acetate was 70.41% and 61.19%, and the hydrophobicity of the isolates towards xylene and n-hexane was between 30% and 40%. The two isolates could inhibit the growth Escherichia coli, Hafnia alvei, Staphylococcus saprophyticus, Serratia marcescens, and Staphylococcus equorum. The antioxidant capacity of PC7-C11 was superior to that of PC7-C6, with a 59.33% scavenging rate of 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 53.49% scavenging rate of hydroxyl radicals respectively. The enzymatic characteristics of PC7-C11 showed that the optimal reaction temperature and pH for enzyme activity were 40 ℃ and pH 7. Ethanol, glucose, dimethyl sulfoxide, SDS, EDTA, Fe2+, Mg2+, Na+, and Ca2+ inhibited enzyme activity to varying degrees, while K+ has a certain promoting effect on enzyme activity.

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檀茜倩,程笑笑,崔方超,王当丰,吕欣然,俞张富,沈荣虎,励建荣.泡菜中产β-葡萄糖苷酶植物乳杆菌的益生和酶学特性[J].中国食品学报,2024,24(9):69-79

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  • 收稿日期:2023-09-12
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  • 在线发布日期: 2024-10-21
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