抑制活菌泡菜贮藏过程中品质劣变的研究
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(浙江工商大学食品与生物工程学院 杭州 310018)

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浙江省公益技术研究计划农业项目(LGN18C 200014);浙江省教育厅科研项目(Y202250368)


Studies on the Inhibition of the Gas Production and Quality Deterioration of Pickles during Storage
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(School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018)

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    摘要:

    目的:研究活菌泡菜产品在贮藏流通过程中由微生物引起的产品品质变化,开发延长泡菜贮藏期的新方法。方法:对从泡菜中分离鉴定出来的8种乳酸菌,在不同pH培养基条件下检测各种乳酸菌生长速度。对8种乳酸菌进行产气测定。在发酵前添加泡菜汁、嗜酸乳杆菌、植物乳杆菌、木糖醇等,在不同温度条件下发酵和贮藏,对泡菜的色泽进行评分,对酸度、混浊度、挥发性物质进行测定。结果:在pH 4.5时,植物乳杆菌是优势菌,其次是短乳杆菌。在pH 4.0~3.0时,嗜酸乳杆菌是优势菌,其次是植物乳杆菌,主要产气菌是短乳杆菌、假肠膜明串珠菌、坏发酵乳杆菌。pH 4.0~4.5时,主要产气菌是短乳杆菌。在pH 3.5时,主要是坏发酵乳杆菌产气。在pH 3.0时,不容易产气。发酵前添加2 mL/L 1×105 CFU/mL植物乳杆菌和20 mg/g木糖醇,可显著延长常温和低温下泡菜的贮藏期和货架期。结论:1×105 CFU/mL植物乳杆菌和20 mg/g木糖醇处理,显著抑制常温贮藏泡菜的总挥发物积累,抑制泡菜的异硫氰酸烯丙酯、萘类积累,有利于维持原初气味特征。

    Abstract:

    Objective: The quality changes caused by microorganisms in the process of pickle storage and circulation were investigated and a new method to prolong the storage life of pickle was developed. Methods: The growth rate of 8 lactobacillus from pickles was tested under different pH media and their gas production was measured. Before cabbage fermentation, adding pickled liquid, Lactobacillus acidophilus, Lactobacillus plantarum, xylitol, etc., The pickle was fermented and stored at ambient and low temperatures for 31 d and 73 d respectively. The color of pickle was scored, and acidity and turbidity and volatile substances were determined. Result: Lactobacillus plantarum was the dominant bacterium, followed by Lactobacillus brevis when 8 lactobacillus grow on pH 4.5 culture medium. Lactobacillus acidophilus is the dominant bacterium, followed by Lactobacillus plantarum of 8 lactobacillus on pH 4.0 to 3.0 culture medium. Lactobacillus brevis, Leuconostoc pseudoentericus, and Lactobacillus fermentans all contributed to gas production during pH 4.0 to 3.0, and gas production mainly caused by Lactobacillus brevis during pH 4.0 to 4.5, caused by Lactobacillus malefermentans at pH 3.5, while gas production was greatly inhibited at pH 3.0. The addition of 2 mL/L 1×105 CFU/mL Lactobacillus plantarum combined with 20 mg/g xylitol significantly prolonged the storage and shelf life of the pickles at ambient and low temperature. Conclusion: The treatment with 1×105 CFU/mL Lactobacillus plantarum combined with 20 mg/g xylitol significantly inhibited the accumulation of total volatiles, allyl isothiocyanate, and naphthalene in pickles, which contributed to the maintenance of the original odor characteristics.

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王向阳,盛雪蓉,俞兴伟,施永清,顾双.抑制活菌泡菜贮藏过程中品质劣变的研究[J].中国食品学报,2024,24(9):389-397

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  • 收稿日期:2023-09-16
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  • 在线发布日期: 2024-10-21
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