富含蛋白质浓稠蛹虫草乳的流变学和体外消化特性研究
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(1.山西农业大学食品科学与工程学院 山西太谷 030801;2.山西省黄土高原食用菌重点实验室 山西太谷 030801;3.山西省食用菌工程技术研究中心 山西太谷 030801)

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山西省科技重大专项计划“揭榜挂帅”项目(202301140601015);山西省现代农业产业技术体系建设专项(2023CYJSTX09);山西省基础研究计划(自由探索类)项目(20210302123384)


Study on Rheology and in Vitro Digestion of Dense Cordyceps militaris Milk Rich in Protein
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(1.College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, Shanxi;2.Shanxi Key Laboratory of Edible Fungi on Loess Plateau, Taigu 030801, Shanxi;3.Shanxi Engineering Research Center of Edible Fungi, Taigu 030801, Shanxi)

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    摘要:

    为研究富含蛋白质浓稠蛹虫草乳的流变学特性及体外消化特性,在蛹虫草乳中分别添加乳清分离蛋白(WP)或大豆分离蛋白(SPI)使蛹虫草乳中蛋白含量在10%以上,加入增稠剂黄原胶(XG)、卡拉胶(KC)或羧甲基纤维素钠(CMC),测定其流动性、黏弹性和消化特性。结果表明,所有样品都表现出弱凝胶行为,表观黏度(η):CM-WP-XG(1.986 Pa·s)>CM-SPI-CMC(1.826 Pa·s)>CM-SPI-XG(1.548 Pa·s)>CM-SPI-KC(1.201 Pa·s);凝胶强度(G0′-G0′′)和复合模量(η):CM-SPI-XG(996.3和121.39 Pa·s)>CM-SPI-CMC(613.41和56.80 Pa·s)>CM-SPI-KC(290.62和34.11 Pa·s)>CM-WP-XG(93.02和8.82 Pa·s);损耗系数(Tanδ):CM-WP-KC(0.66)>CM-WP-CMC(0.61)>CM-SPI-KC(0.44)>CM-SPI-CMC(0.42)。通过体外消化发现,CM-SPI-CMC(0.3096%)和CM-SPI-XG(0.3033%)游离氨基酸含量较高,同时通过对G和Tanδ比较,高G和低Tanδ的蛹虫草乳更适合吞咽困难人群。根据以上比较,CM-SPI-XG和CM-SPI-CMC具有良好的弹性和抗变形性,更有利于吞咽困难和老年人食用。综合试验分析,CM-SPI-XG是适合中度吞咽困难患者或老年人食用的最佳组合。这项研究表明蛋白质-增稠剂相互作用,食物基质影响流变学和消化特性,研究结果为吞咽困难和老年人的饮食需求提供一定的理论支持。

    Abstract:

    In order to study the rheological and in vitro digestive properties of protein-rich dense Cordyceps militaris milk, whey protein isolate (WP) or soybean protein isolate (SPI) were added to make the protein content of Cordyceps militaris milk more than 10%, and thickening agents Xanthan gum (XG), Kappa-Carrageenan (KC) or Carboxymethylcellulose sodiu (CMC) were added. The fluidity, viscoelasticity and digestive properties were determined. The results showed that all samples exhibited weak gel behavior, apparent viscosity (η): CM-WP-XG (1.986 Pa·s) > CM-SPI-CMC (1.826 Pa·s) > CM-SPI-XG (1.548 Pa·s) > CM-SPI-KC (1.201 Pa·s); Gel strength(G0′-G0″) and compound modulus (η): CM-SPI-XG (996.3 and 121.39 Pa·s) > CM-SPI-CMC (613.41 and 56.80 Pa·s) > CM-SPI-KC (290.62 and 34.11 Pa·s) >CM-WP-XG (93.02 and 8.82 Pa·s); Loss coefficient (Tanδ): CM-WP-KC (0.66) > CM-WP-CMC (0.61) >CM-SPI-KC (0.44) >CM-SPI-CMC (0.42). In vitro digestion showed that CM-SPI-CMC (0.3096%) and CM-SPI-XG (0.3033%) had higher content of free amino acids. Meanwhile, by comparing G and Tanδ, Cordyceps militaris milk with high G and low Tanδ was more suitable for people with dysphagic difficulty. According to the above comparison, CM-SPI-XG and CM-SPI-CMC had good elasticity and deformation resistance. More conducive to dysphagia and the elderly eat. In conclusion, CM-SPI-XG is the best combination suitable for patients with moderate dysphagia or the elderly. This study demonstrates how protein-thickener interactions and food substrates determine the rheological and digestive properties of samples, providing theoretical support for dysphagia and dietary needs of the elderly.

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魏世杰,苏翠馨,常明昌,孟俊龙,冯翠萍,曹谨玲,云少君,徐丽婧,耿雪冉,程菲儿,程艳芬.富含蛋白质浓稠蛹虫草乳的流变学和体外消化特性研究[J].中国食品学报,2024,24(10):105-114

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  • 收稿日期:2023-10-05
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  • 在线发布日期: 2024-12-16
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