To study the effect of electric field treatment on oxidation stability of soybean crude oil. Based on the influence of single factor about electric field intensity or treatment time on lipid peroxide value (POV), the optimal conditions of electric field treatment were determined. The content changes of sterol, squalene and tocopherol under the optimal electric field condition were determined by GC-MS and HPLC. The antioxidant capacity of oils with the electric field treatment was evaluated in vitro. The optimal electric field intensity was 3 000 V/m and the electric field treatment time was 3 h. The relative loss inhibition rate of sterol, squalene and tocopherol were 23.08% and 19.99%, respectively. The loss inhibition rate of sterol and squalene were different for tocopherol. The protection order was δ-tocopherol > β-tocopherol > α-tocopherol > γ-tocopherol, and the electric field had a relatively obvious effect on the inhibition rate of δ-tocopherol loss in soybean crude oil. Electric field treatment slowed down the decrease rate of DPPH and ABTS+ free radical scavenging activity and FRAP reducing ability of oils, and protected the antioxidant capacity of oils. Electric field can inhibit the oxidation rate and the degree of oxidation of oils, and has an inhibitory effect on the loss of antioxidant components in oils, which protects the antioxidant capacity of oils and has a good antioxidant effect.