分离自泡菜中的1株高产胞外多糖植物乳杆菌的益生特性
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(1.渤海大学食品科学与工程学院 辽宁锦州 121013;2.杭州萧山农业发展有限公司 杭州 311215)

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云南省科技人才与平台计划项目(202205AF150060)


Probiotic Characteristics of Lactobacillus plantarum with High Yield of Exopolysaccharides (EPS) Isolated from Pickled Vegetables
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(1.College of Food Science and Engineering, Bohai University, Jinzhou 121013, Liaoning;2.Hangzhou Xiaoshan Agriculture Development Co., Ltd., Hangzhou 311215)

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    摘要:

    对泡菜中产胞外多糖的乳酸菌进行筛选,对一株胞外多糖产量最高的菌株PC715进行鉴定,并分析了其生长、耐酸和耐胆盐能力、自聚集性、共聚集性、疏水性、安全性、抗菌活性和体外抗氧化能力等益生特性。结果表明,此菌株为植物乳杆菌,胞外多糖EPS715产量约为870 mg/L。紫外-可见光谱表明EPS715中不存在蛋白质或核酸。植物乳杆菌PC715在28 ℃和37 ℃均能良好生长,在酸性条件下(pH 2.5,3.0和4.0)存活率分别为75.54%,91.77%和93.69%,在胆盐(1,2,3 mg/mL)中存活率高于40%,自聚集率约为27.64%,对大肠杆菌的共聚集率约为16%,对不同有机溶剂的疏水能力在0.88%~16.97%之间。植物乳杆菌PC715对四环素、氨苄青霉素、红霉素、氯霉素和克林霉素敏感,无溶血性;可在一定程度上抑制大肠杆菌、蜡样芽孢杆菌、腐生葡萄球菌、蜂房哈夫尼亚菌、金黄色葡萄球菌和不动杆菌的生长;PC715无细胞上清液对DPPH、羟自由基和ABTS+自由基的清除能力分别为(62.90±0.87)%,(93.39±2.20)%和(60.19±1.07)%。本研究表明,所分离产胞外多糖植物乳杆菌PC715具有一定益生潜力,为其在发酵中的应用提供一定的理论依据。

    Abstract:

    In the study, lactic acid bacteria which could produce exopolysaccharids (EPS) were screened from pickled vegetables, the yield of the EPS was determined, and the strain PC715 which have the highest EPS producing ability was identified, at the mean time, the probiotic characteristics of PC715 were also evaluated, including its growth status, acid and bile salt tolerance, self-aggregation, co-aggregation, hydrophobicity, safety, antibacterial activity, and in vitro antioxidant capacities. The results showed that based on the 16S rDNA sequence, the strain with the highest EPS production yield (PC715) was identified as Lactobacillus plantarum. The yield of EPS715 was about 870 mg/L detected by the phenol-sulfuric method. The ultraviolet-visible spectrum indicated no proteins or nucleic acids were in EPS715. L.plantarum PC715 could grow well at both 28 ℃ and 37 ℃, its survival rates at acid conditions (pH=2.5, 3.0, and 4.0) were 75.54%, 91.77%, and 93.69%, respectively, and its survival rates in bile salts (1, 2, and 3 mg/mL) were higher than 40%. The self-aggregation rate of the stain was about 27.64%, the co-aggregation towards Escherichia coli was at about 16%, and the hydrophobic ability were between 0.88%-16.97% towards different organic solvents. L.plantarum PC715 were sensitive to tetracycline, ampicillin, erythromycin, chloramphenicol, and clindamycin, had no hemolytic activity, and had inhibitory effects on E.coli, Bacillus cereus, Staphylococcus saprophyte, Hafnia alvei, Staphylococcus aureus, and Acinetobacter sp. The scavenging capacity of DPPH, hydroxyl, and ABTS+ radicals of L.plantarum PC715 cell-free supernatant were (62.90±0.87)%, (93.39±2.20)%, and (60.19±1.07)%, respectively. This study showed that exopolysaccharides producing-L.plantarum PC715 might had probiotic potential and could be used as the starter culture in the fermentation industry.

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檀茜倩,麻冰玉,程笑笑,崔方超,王当丰,俞张富,沈荣虎,励建荣.分离自泡菜中的1株高产胞外多糖植物乳杆菌的益生特性[J].中国食品学报,2024,24(11):22-31

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  • 收稿日期:2023-11-24
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  • 在线发布日期: 2024-12-25
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