黑果腺肋花楸多酚对鱼糜凝胶特性的影响
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(1.河北科技师范学院 食品科技学院 河北昌黎 066600;2.河北省果品加工技术创新中心 河北昌黎 066600)

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河北省“三三三人才工程”资助项目(B20221006);河北省果品加工技术创新中心绩效补助经费项目(225676115H)


Effect of Polyphenols of Aronia melanocarpa on the Gel Properties of Fish Surimi
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(1.College of Food Science and Technology, Hebei Normal University of Science and Technology, Changli 066600, Hebei;2.Hebei Fruit Processing Technology Innovation Center, Changli 066600, Hebei)

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    摘要:

    为了提升淡水鱼鱼糜制品的品质及口感,采用傅里叶红外光谱、低场核磁共振及扫描电镜等方法探究黑果腺肋花楸多酚添加量对鱼糜凝胶特性的影响,并测定鱼糜凝胶持水性、全质构、化学作用力、总巯基含量、弛豫时间、蛋白质二级结构及扫描电镜等。结果表明,当多酚添加量为0.375%时,鱼糜凝胶的持水性、凝胶特性、弹性、咀嚼性最好;弛豫谱中该组谱峰最高,向左偏移最多,说明多酚添加量为0.375%的鱼糜凝胶中不易流动水最多,结构稳定。扫描电镜中,随着多酚添加量的增加,鱼糜凝胶结构逐渐致密,0.375%组的鱼糜凝胶结构最致密,表面光滑;当多酚添加量增至0.500%时,鱼糜凝胶结构变得松散。化学作用力中二硫键逐渐增多且与总巯基含量呈反比,二硫键与疏水相互作用对鱼糜凝胶的形成起重要作用。傅里叶红外光谱表明α-螺旋结构逐渐转变为β-折叠结构,且β-折叠峰面积逐渐增大,表明蛋白质二级结构变得规则、有序,使鱼糜凝胶特性增强。十二烷基硫酸钠聚丙烯酰胺凝胶电泳中鱼糜凝胶肌球蛋白重链(MHC)和肌动蛋白(AC)条带逐渐减弱,表明形成稳定的鱼糜凝胶网络结构。

    Abstract:

    In order to improve the quality and taste of freshwater fish surimi products, fourier transform infrared spectroscopy, low field nuclear magnetic resonance and scanning electron microscopy were used to explore the effects of different levels of Aronia melanocarpa polyphenols on surimi gel properties, and the water retention, whole texture, chemical force, total sulfhydryl content, relaxation time, protein secondary structure and scanning electron microscopy of surimi gel were measured. The results showed that when the content of polyphenols was 0.375%, surimi gel had the best water holding capacity, gel properties, elasticity, and chewability. In the relaxation spectrum, the peak of the group with 0.375% of polyphenols was the highest and shifted to the left the most, indicating that the surimi gel with 0.375% of polyphenols had the most difficult flowing water and promoted structural stability. In the scanning electron microscope, the structure of surimi gel became denser with the increase of polyphenol addition. The surimi gel in 0.375% group was the densest with smooth surface. When the amount of polyphenol added increased to 0.500%, the structure of surimi gel became loose again. The disulfide bond in the chemical force gradually increased and was inversely proportional to the total sulfhydryl content. The disulfide bond hydrophobic interaction played an important role in surimi gel. Fourier transform infrared spectroscopy indicated that alpha helical structure gradually transformed into a beta folded structure, and the area of the beta folded peak gradually increased indicating that the protein secondary structure of the protein was more regular and orderly, which promoted the enhancement of surimi gel properties. In SDS-PAGE, myosin heavy chain (MHC) and actin (AC) of surimi gel gradually weakened, indicating that a stable surimi gel network structure was formed.

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王亚菲,马子龙,李军,朱凤妹.黑果腺肋花楸多酚对鱼糜凝胶特性的影响[J].中国食品学报,2024,24(11):269-278

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  • 收稿日期:2023-11-24
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  • 在线发布日期: 2024-12-25
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