Objective: To isolate and purify probiotic Lactobacillus from honey with high adsorption rate of heavy metal lead and edible. Methods: Physiological and biochemical experiments were performed on the isolated and purified strains， and a phylogenetic tree based on 16s rDNA was established for taxonomic identification； the probiotic properties of the strains were assessed by hemolysis， gelatinase activity， nitrate reduction and amino acid decarboxylase， self-aggregation and hydrophobicity， acid and bile salt resistance， and antimicrobial drug sensitivity. Meanwhile， inductively coupled plasma emission spectroscopy was used to determine the Pb2+ content in the solution before and after adsorption. The ability of the strains to adsorb heavy metals was explored. Scanning electron microscopy-X-ray energy spectroscopy was used to observe the adsorption of Pb2+ by the bacteria before and after adsorption. Fourier transform infrared spectroscopy was used to determine the adsorption of Pb2+ by the bacterium and the changes in protein structure. Results: Strain C10 screened from honey was identified as Lactobacillus plantarum， with no hemolytic and gelatinase activities； it could not reduce nitrate and did not decarboxylate to produce amines； self-aggregation and hydrophobicity reached 85.33% and 35.50%， respectively； survival under acid and bile salts was 39.39% and 98.79%， respectively； and it had strong sensitivity to chloramphenicol， erythromycin， ampicillin and cotrimoxazole. Lactobacillus plantarum C10 adsorbed 65.82% of heavy metals within 2 h. Scanning electron microscopy-X-ray spectroscopy showed that the percentage of lead element was 9.69%， and Fourier transform infrared spectroscopy showed that the secondary structure of the surface protein of the bacterium changed significantly after the adsorption of Pb2+. Conclusion: Lactobacillus plantarum C10 has good probiotic properties， can effectively adsorb Pb2+， and has the effect of preventing heavy metal poisoning.