Ovalbumin (OVA) is one of the main allergens that cause egg allergy， and pulsed electric field (PEF) is an emerging non-thermal physical processing technology that has an ameliorative effect on OVA-induced allergic reactions. Based on the BALB/c mouse model， the effects of PEF-assisted alcalase (AL) treatment on OVA-induced allergic reactions were systematically evaluated by body weight， body composition， allergy symptoms， histopathology， antibody levels， and inflammatory mediators release levels. The results showed that PEF-assisted AL treatment ameliorated the body composition changes caused by OVA-induced allergic reactions by improving body weight， lean content and free water content in mice. The results of clinical symptoms showed that PEF-assisted AL treatment improved the problems of hypertrophy and increased vascular permeability caused by allergic reactions， and significantly improved the problems of body temperature drop caused by allergic reactions. The histopathological results showed that PEF-assisted AL treatment significantly improved the inflammatory infiltration of spleen and lung， and made their morphology more similar to that of the control group. Serological results showed that， compared with OVA group， the binding levels of specific IgE and IgG1 in HOVA-PP' group were decreased by 68.87% and 31.20%， respectively， indicating that PEF-assisted AL treatment significantly reduced the binding levels of allergens and specific antibodies. In addition， PEF-assisted AL treatment significantly reduced the concentration of histamine and mouse mast cell protease-1 (mMCP-1) to 51.97 ng/mL and 51.97 pg/mL， regulated the release of IL-4 and IFN-γ， and promoted the balance between Th1 and Th2-type cells， thereby improving OVA-induced allergic reactions.