Based on the multiomics detection technology of human milk developed by the China maternal and infant nutrition health cohort study， the lipidomics， glycomics and proteomics of human milk and different brands of infant formula were compared and analyzed， and the differences in the important nutritional composition of simulated human milk of infant formula were explored. The species number of glycerides (94)， phospholipids (224)， oligosaccharides (152) and milk fat globule membrane protein (1 043) in human milk were significantly higher than those of infant formula (P<0.05). The contents of phospholipids (256.77 mg/L)， oligosaccharides (10 150.60 mg/kg) and total sialic acid (521.30 mg/100 g) in infant formula were significantly different from those in human milk(P<0.05). The species and abundance of milk fat globule membrane proteins of infant formula and human milk were quite different， and only 10 kinds of human milk fat globule membrane proteins were reduced. In the future， the development of infant formula not only needs to simulate the richness of important nutritional molecules， but also needs to increase its content accordingly to bring it closer to the composition of human milk.