Objective: To investigate the effect of abiotic induction of germination on the enrichment of resveratrol (RES) in peanut， and to determine the protective effect of RES on the oxidative damage of HEK293T cells under the optimal enrichment conditions. Methods: Light type， ultrasound intensity， copper sulphate concentration and germination time were used as univariate conditions to induce peanut germination， and the effects of abiotic induction of germination on RES and the optimal enrichment conditions were investigated based on univariate and orthogonal experiments. The highest toxicity of RES on HEK293T cells was determined by cell viability assay. The protective effect of RES against H2O₂-induced antioxidant damage in HEK293T cells was investigated by measuring intracellular reactive oxygen species (ROS) content， cellular superoxide dismutase (SOD) activity and catalase (CAT) activity. Results: Light， ultrasound and copper sulphate were all effective in promoting resveratrol content in germinated peanuts， and the combined germination showed a promoting effect on resveratrol enrichment. The optimal parameters for resveratrol enrichment in peanut buds were: Blue light irradiation， ultrasound intensity of 120 W/cm3， CuSO4 concentration of 0.6 mmol/L， and germination for 3 d. At this time， the resveratrol content of peanut buds was 35.79 μg/g. RES intervention treatment significantly restored the viability of H2O₂-induced oxidative damage-producing HEK293T cells， reduced the production of intracellular ROS， and increased the viability of SOD， CAT viability. Conclusion: Multiple germination such as light could enrich resveratrol in germinated peanuts， and RES has a significant protective effect on cells against oxidative stress damage.