In order to clarify the research hotspots and development trends of peptide self-assembly at home and abroad， 188 papers related to peptide self-assembly published on CNKI from January 1993 to July 2023 and 13 807 papers related to peptide self-assembly published on WoS core collection were used as samples. CiteSpace was used to draw visual maps of institutions， authors and Keywords， and statistical information was obtained for analysis and discussion. Studies had shown that peptide self-assembly research was currently in a stage of rapid development， and various institutions in the field were constantly exploring new research directions. At present， there were many core authors in this field. The research in the field of peptide self-assembly in China started late， and the cooperation ability between scholars and research institutions engaged in peptide self-assembly research was generally weaker than that of foreign scholars and research institutions. The research hotspots mainly focus on the preparation and application of peptide self-assembled hydrogels， the preparation of self-assembled nanomaterials， and the synthesis of polypeptides. Self-assembled antimicrobial peptides， self-assembled anti-tumor peptides and other less studied directions might become future research hotspots.