魔芋葡甘聚糖对热诱导米糠蛋白凝胶性能、微观结构及其分子力的影响
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(1.黑龙江八一农垦大学食品学院 黑龙江大庆 163319;2.粮食副产物加工与利用教育部工程研究中心 黑龙江大庆 163319;3.黑龙江省北大荒米业集团有限公司 哈尔滨 150090)

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国家重点研发计划项目(2021YFD2100904); 黑龙江省“双一流”学科协同创新成果项目(LJGXCG2024-P32)


Effects of Konjac Glucomannan on the Gel Properties, Microstructure and Molecular Forces of Heat-induced Rice Bran Protein Gels
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(1.College of Food, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang;2.Engineering Research Center of Processing and Utilization of Grain Byproducts, Ministry of Education,Daqing 163319, Heilongjiang;3.Heilongjiang Beidahuang Rice Industry Group Co., Ltd., Harbin 150090)

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    摘要:

    以米糠蛋白(RBP)和魔芋葡甘聚糖(KGM)为原料,采用热诱导方法制备RBP凝胶及RBP-KGM复合凝胶。通过流变特性、傅里叶红外光谱、荧光光谱和扫描电子显微镜图片等方法探究KGM浓度对热诱导RBP凝胶性能和结构特征的影响。结果表明:总复合凝胶质量分数6%时,随着KGM质量分数的增加,复合凝胶表观黏度增加,当KGM质量分数超过20%时,储存模量G'始终大于损耗模量G″,表现出黏弹性行为且应变值γmax也由0.099%减至0.015%,凝胶的应变硬化程度增加,RBP-KGM复合凝胶由溶胶向更接近纯弹性固体转变。内源荧光光谱显示KGM对RBP具有静态猝灭作用,热力学参数为?驻G<0,?驻H>0,?驻S>0,RBP-KGM复合凝胶相互作用方式为疏水相互作用主导。KGM质量分数由0%增至33.3%时,氢键、疏水相互作用分别由(2.16±0.14)%,(4.13±0.14)%升至(2.63±0.10)%,(6.82±0.07)%,初始分解温度提高到359.15 ℃,相对结晶度指数最大为(50.00±1.00)%。RBP-KGM复合凝胶中T2弛豫时间最短且固定水含量高,其微观结构说明加入KGM可以通过增强分子间相互作用,使该复合凝胶深度聚集,并形成紧密堆积的复杂凝胶网络。本研究为RBP-KGM复合凝胶的制备及应用提供理论依据。

    Abstract:

    In this study, RBP gels and RBP-KGM composite gels were prepared by heat-induced method using rice bran protein and konjac glucomannan as materials. The effects of konjac glucomannan concentration on the gel properties, structural properties and microstructure of heat-induced rice bran protein gels were investigated by rheological properties, Fourier transform infrared spectroscopy, fluorescence spectroscopy and scanning electron microscopy images. The results showed that when the total composite gel concentration was 6%, the apparent viscosity of the composite gel increased with the increasing of KGM concentration. When the concentration of konjac glucomannan was exceed 20%, the storage modulus G' was always exceed than the loss modulus G'', which showed viscoelastic behavior and the strain value γmax also decreased from 0.099% to 0.015%, the strain hardening degree of the gel increased, and the composite gels transformed from sol to a pure elastic solid. Fluorescence spectroscopy showed that konjac glucomannan had a static quenching effect on rice bran protein, and the thermodynamic parameters were ?驻G<0, ?驻H>0, ?驻S>0, and the interaction mode of the composite gel was dominated by hydrophobic interaction. When the concentration of konjac glucomannan increased from 0.00% to 33.30%, the hydrogen bond and hydrophobic interaction increased from (2.16±0.14)%, (4.13±0.14)% to (2.63±0.10)%, (6.82±0.07)%, respectively. The initial decomposition temperature was increased to 359.15 ℃, the relative crystallinity index was (50.00±1.00)%, and the T2 relaxation time in the composite gel was the shortest and the fixed water content was high. The microstructure further suggests that the addition of konjac glucomannan can enhance the intermolecular interaction, make the composite gels deeply aggregate, and form a tightly packed complex gel network. This study provided a theoretical basis for the preparation and application of RBP-KGM composite gels, and provided a reference for the deep processing of rice bran and the industrial application of RBP.

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李琳,高永娇,张智,孙嘉辉,孙立兰,左锋.魔芋葡甘聚糖对热诱导米糠蛋白凝胶性能、微观结构及其分子力的影响[J].中国食品学报,2025,25(3):123-135

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  • 收稿日期:2024-03-27
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  • 在线发布日期: 2025-05-23
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