Insomnia is getting worse with increasing social pressures， and the number of insomnia patients has increased sharply. The natural melatonin in milk has a certain effect on improving sleep. In this study， a mouse model of insomnia was established by sleep deprivation， and was used for field test and sleep induction test， and a clinical evidence-based study was carried out to explore the effects of milk rich in native melatonin (100 pg/mL) on insomnia. The results showed that milk rich in native melatonin shortened the sleep latency of mice from (5.2±0.45) min to (1.6±0.55) min (P<0.05)， and the sleep duration from (115.75±6.5) min to (48.4±3.05) min (P<0.05 )， improving the sleep quality. Compared with the control group， the activity time of the mice in the central area of the open field in the experimental group increased from (11.34±11.36) s to (34.9±29.09) s (P<0.05)， reducing the anxiety level of the mice. The scores of the sleep quality scale for people with insomnia dropped from (14.93±3.70) points to (10.88±3.96) points (P<0.05)， and the insomnia severity scale score dropped from (12.25±3.26) points to (8.81±2.93) (P<0.05) points. The intervention of native melatonin-rich milk can improve the sleep quality of insomnia population， and the level of insomnia and daytime sleepiness was reduced for people with insomnia.