In order to develop a rapid method for the chemical analysis and quality evaluation of R. roxburghii， the dried matrix spot of R. roxburghii was prepared. The dried matrix spot of R. roxburghii were placed within an online filter， connected to high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) for rapid analysis of chemical components， and the fingerprint profile was established. The results showed that 39 compounds were identified in R. roxburghii juice， and 48 compounds were identified in the pomace. The metabolic profiles of the juice and pomace showed high similarity. The HPLC fingerprint of R. roxburghii juice based on dried matrix spots showed 10 common peaks. The similarity between 15 batches of R. roxburghii juice was greater than 0.97. The use of dried matrix spots effectively addresses issues of sample instability during quality analysis， as well as challenges in transportation and storage， providing a reference for the rapid evaluation of R. roxburghii products.