纳米纤维素抗冻活性及其改善冷冻鱼糜品质研究
CSTR:
作者:
作者单位:

(1.西南大学食品科学学院 重庆 400715;2.宜宾西南大学研究院 四川宜宾 644000;3.重庆市农业科学院 重庆 401329)

作者简介:

通讯作者:

中图分类号:

基金项目:

“十四五”国家重点研发计划项目(2023YFD 2100205);国家自然科学基金项目(32372353);重庆市农业科技攻关项目(NW-CSTB2025 NYKJGGXM-NCPJGLXMX0003);四川省自然科学基金项目(2025ZNSFSC1058)


Studies on the Antifreeze Activity of Nanocellulose and Its Improvement of the Quality of Frozen Surimi
Author:
Affiliation:

(1.College of Food Science, Southwest University, Chongqing 400715;2.Yibin Academy of Southwest University, Yibin 644000, Sichuan;3.Chongqing Academy of Agricultural Sciences, Chongqing 401329)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:评估纤维素纳米晶体(CNC)作为新型抗冻剂改善冷冻鱼糜品质的潜力。方法:采用滴溅法测定CNC的冰重结晶抑制(IRI)活性。将不同质量分数CNC(1%,3%,5%)添加至草鱼鱼糜中,在不同冻藏时间(0,10,20,30,40 d)下测定肌原纤维蛋白(MP)含量、SDS-PAGE图谱、总巯基含量及持水性。通过动态流变分析评估鱼糜凝胶的黏弹性变化。结果:CNC表现出优异的IRI活性和剂量效应,0.5% CNC处理组的冰晶尺寸集中在14~20 μm,冰晶大小分布均匀,而阴性对照组的冰晶多集中在100~120 μm。冻藏40 d后,5% CNC组鱼糜的MP含量、总巯基含量和持水性分别保持在2.43 mg/mL,48.14 μmol/g,80.54%,均显著高于NC组(1.39 mg/mL,31.10 μmol/g,68.27%)(P < 0.05),表明其能够有效延缓冷冻鱼糜冻藏期间的品质劣化。鱼糜凝胶的流变性能明显优于对照组,储能模量(G′)显著提高。结论:CNC通过抑制冰晶生长和重结晶,保护蛋白质结构与官能团完整性,有效减缓鱼糜蛋白冷冻变性,改善其保水性与凝胶性能,具有作为高效、无热量、非甜味抗冻剂的应用前景。

    Abstract:

    Objective: To evaluate the potential of cellulose nanocrystals (CNC) as a novel cryoprotectant for improving the quality of frozen surimi. Methods: The ice recrystallization inhibition (IRI) activity of CNC was determined using the splat assay. Different mass fractions of CNC (1%, 3%, 5%) were added to grass carp surimi. The myofibrillar protein (MP) content, SDS-PAGE pattern, total sulfhydryl content, and water-holding capacity were measured at various frozen storage times (0, 10, 20, 30 d, and 40 d). The viscoelastic properties of surimi gels were evaluated by dynamic rheological analysis. Results: CNC exhibited excellent IRI activity with a dose-dependent effect. In the 0.5% CNC treatment group, ice crystal sizes were concentrated in the range of 14-20 μm with a uniform distribution, whereas ice crystals in the negative control group were mostly concentrated in the range of 100-120 μm. After 40 d of frozen storage, the MP content, total sulfhydryl content, and water-holding capacity of surimi in the 5% CNC group remained at 2.43 mg/mL, 48.14 μmol/g, and 80.54%, respectively, all significantly higher than those in the NC group (1.39 mg/mL, 31.10 μmol/g, and 68.27%) (P < 0.05), indicating that CNC effectively delayed the quality deterioration of frozen surimi during storage. The rheological properties of surimi gels were significantly better than those of the control group, with markedly increased storage modulus (G′) and gel strength. Conclusion: By inhibiting ice crystal growth and recrystallization, CNC protects the structural and functional integrity of proteins, effectively alleviates protein denaturation in frozen surimi, and improves its water-holding capacity and gel properties. CNC thus shows promise as an efficient, calorie-free, non-sweet cryoprotectant.

    参考文献
    相似文献
    引证文献
引用本文

王雨西,向思如,张宇昊,戴宏杰.纳米纤维素抗冻活性及其改善冷冻鱼糜品质研究[J].中国食品学报,2026,(4):249-258

复制
分享
相关视频

文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2025-09-04
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2026-06-12
  • 出版日期:
文章二维码
版权所有 :《中国食品学报》杂志社     京ICP备09084417号-4
地址 :北京市海淀区阜成路北三街8号9层      邮政编码 :100048
电话 :010-65223596 65265375      电子邮箱 :chinaspxb@vip.163.com
技术支持:北京勤云科技发展有限公司

漂浮通知


×
喜报 | 《中国食品学报》入选2025年度首都科技期刊卓越行动计划中英文单刊