Loquat flowers are by-product of loquat cultivation. They arerich in bioactive components such as flavonoids and have significant development potential. In this study， ultrasound-assisted extraction technology was used， and the total flavonoid extraction amount was taken as the indicator. Single-factor experiments were conducted to investigate the effects of solvent type， ethanol volume fraction， solid-to-liquid ratio， extraction time， and temperature. Response surface methodology (Box-Behnken design) was then applied to optimize the key process parameters. The in vitro antioxidant activity of the crude extract was evaluated using DPPH and hydroxyl radical scavenging assays. Five types of macroporous resins (AB-8， D101， HPD400， NKA-II， and HP-20) were systematically compared in terms of static adsorption and desorption performance. The best resin was selected， and the dynamic purification parameters were optimized. The results showed that the optimal extraction conditions were as follows: Ethanol volume fraction 60%， solid-to-liquid ratio 52∶1， extraction temperature 69 ℃， and extraction time 62 min. Under these conditions， the total flavonoid extraction amount reached 129.80 mg/g， relative standard deviation (RSD) = 1.95%. The crude extract exhibited significant scavenging activity against both DPPH· and ·OH. AB-8 resin demonstrated the best overall performance. The optimal dynamic purification parameters were a sample loading flow rate of 1.0 mL/min， elution with 70% ethanol at a flow rate of 1.0 mL/min， and an eluent volume of 80 mL. After purification， the relative content of total flavonoids increased to (74.47±1.89)%. HPLC analysis revealed that the main components were hyperoside， isoquercitrin， and quercetin glycoside. This study established an efficient and stable integrated process for the extraction and purification of flavonoids from loquat flowers. It provided technical support for the high-value utilization of loquat flower resources and the further development of their active components.