The purification conditions of red raspberry seed flavonoids by polyamide was studied， also identified its activity of vitro hypolipidemic. On the basis of the purification of AB-8 macroporous resin， polyamide was used for secondary purification， then evaluated its ability of bile salt-binding. The optimal purification conditions of polyamide resin as followed： sample concentration was 5.5 mg/mL， sample volume was 100 mL， the sample flow rate was 2.0 mL/min and sample pH was 4.5. Then the volume fraction of the ethanol was 60%， the volume of elution was 150 mL， and the flow rate of elution was 1.5 mL/min. The purity of red raspberry seed flavonoids was 48.19% and increased by 23.12%. The half maximal inhibitory concentration of flaconoids in binding sodium cholate sodium taurocholate and glycocholate was 2.911， 3.779， 3.257 mg/mL. Thus red raspberry seed flavonoids have good binding capacity with bile salts and are a natural herb with hypolipidemic activities.