Changes on the content of abscisic acid （ABA）， gibberellins（GA）， indole-3-acetic acid（IAA） and zeatin nucleoside （ZR） in different tissues of Agaricus bisporus （Lange Sing） mushroom during 20 ℃ storage were investigated in this study. Results showed that the change pattern of GA and IAA content is similar during postharvest A. bisporus mushroom storage. GA and IAA may synergistically control the processes of cap expansion， protecting veil break and the spore production. Both of ABA and GA play prominent roles in the postharvest senescence process of stipe tissue. ABA may promote and participate in the whole senescence process of harvested A. bisporus fruit bodies. Compared with ABA， GA and IAA， ZR is distributed obviously in different tissues and may closely relate to the gill thickening and spore production. The above mentioned results indicated that the production of the four hormones were difference in different tissues of the A. bisporus mushroom during postharvest storage， which is probably associated with the postharvest continued development and senescence processes. The discovery in this study will provide a theory base for seeking new methods to effectively maintain postharvest quality of A. bisporus mushroom.