Lycopene-load nanoemulsions were prepared through high-pressure homogenization， with octenyl succinate anhydride modified starch （OSA starch） as emulsifier. Nanoemulsions stability was detected through Turbiscan AGS in 24 h and the influence of different storage and application conditions on this system were investigated. The result showed that Ostwald ripening may be the main factor of Lycopene-load nanoemulsions instability. The storage research showed that the storage conditions had a significant effect on the stability of the system. The influence on the diameter of nanoemulsions followed the sequence： temperature > light > oxygen， while the influence on retention rate of lycopene was followed as oxygen > temperature > light. After 28 days storage at 4 ℃ with a dark and nitrogen environment， the particle diameter increased from 138 nm to 157 nm， and the retaintion rate of lycopene was 41%， which showed a good storage stability. The application research showed that the acid resistances of the lycopene-load nanoemulsions were widely， ionic strength and sucrose could not change the stability of nanoemulsions system.