With the improvement of living standards， people’s demand for food safety has improved continuously， and the drawbacks of unreasonable use of antibiotic has attracted widespread attention. Bacteriocins are ribosomally synthesized proteins or peptides synthesized by numerous bacteria that inhibit or kill other bacteria. It is considered to be the potential substitute for chemical preservatives and antibiotics due to their efficient antimicrobial activity， heat stability no residue， no drug resistance. In present study， an efficient spray drying-based bacteriocin preparation method was developed. Lactobacillus plantarum ZJ316 originally isolated from infant fecal with a broad spectrum of antibacterial activity was used as bacteriocin producing strain. The spray drying conditions including excipient， salt addition ratio， inlet temperature， the working efficiency of fan and others were optimized by single factor and orthogonal test. Our results showed that the optimized spray drying conditions were as follows： salt concentration 20%， inlet air temperature 155 ℃， the air-blower efficiency 100% and the peristaltic pump efficiency. Under these conditions， the yield rate of bacteriocin was 90.235%， the antimicrobial activity of the product was 2 979.291 IU/mL or 2.476×104 IU/g. Together， a spray drying method for the preparation of bacteriocin products was initially established， which promoted the application of bacteriocin products in the food industry.