In order to explore whether egg oil works on gastric ulcer，to establish the mice peptic ulcer model by gavage acetic acid. By detect the ulcer area， Ulcer index， Ulcer inhibition rate， blood and stomach tissue of superoxide dismutase（SOD）， malondialdehyde （MDA）， serum tumor necrosis factor （TNF-α）， stomach tissue nitric oxide （NO）， stomach tissue endothelin-1 （ET-1） of mice， and other indicators to evaluate the effect of egg oil on chronic gastric ulcer. The results showed that： The biochemical indexes of serum and stomach in the high dose group of egg yolk oil were SOD （10.99 ±1.50） U/mg， tissue MDA （0.42 ± 0.04） U/mg， blood SOD （83.23 ± 4.55） U/mg， blood MDA （3.31±1.02） U/mg， tissue ET-1 （7.44 ± 1.52） U/mg， tissue NO （6.83 ± 1.57） U/mg， and tissue TNF-α（4.98 ± 0.87） U/mg compared with the model group， there were significant differences； compared with the normal control group， the indexes showed no significant difference； compared to the positive drug group there was significant difference in blood SOD， tissue NO， and tissue TNF-α. Thus， egg oil has good effect on the healing of digestive gastric ulcer in mice.